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@ARTICLE{Kehrer:619942,
      author       = {Kehrer, Jessica and Pietsch, Emma and Ricken, Dominik and
                      Strauss, Léanne and Heinze, Julia M. and Gilberger, Tim and
                      Frischknecht, Friedrich},
      title        = {{APEX}-based proximity labeling in {P}lasmodium identifies
                      a membrane protein with dual functions during mosquito
                      infection},
      journal      = {PLoS pathogens},
      volume       = {20},
      number       = {12},
      issn         = {1553-7374},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {PUBDB-2024-08033},
      pages        = {e1012788},
      year         = {2024},
      abstract     = {Transmission of the malaria parasite Plasmodium to
                      mosquitoes necessitates gamete egress from red blood cells
                      to allow zygote formation and ookinete motility to enable
                      penetration of the midgut epithelium. Both processes are
                      dependent on the secretion of proteins from distinct sets of
                      specialized vesicles. Inhibiting some of these proteins has
                      shown potential for blocking parasite transmission to the
                      mosquito. To identify new transmission blocking vaccine
                      candidates, we aimed to define the microneme content from
                      ookinetes of the rodent model organism Plasmodium berghei
                      using APEX2-mediated rapid proximity-dependent
                      biotinylation. Besides known proteins of ookinete
                      micronemes, this identified over 50 novel candidates and
                      sharpened the list of a previous survey based on subcellular
                      fractionation. Functional analysis of a first candidate
                      uncovered a dual role for this membrane protein in male
                      gametogenesis and ookinete midgut traversal. Mutation of a
                      putative trafficking motif in the C-terminus affected
                      ookinete to oocyst transition but not gamete formation. This
                      suggests the existence of distinct functional and transport
                      requirements for Plasmodium proteins in different parasite
                      stages.},
      cin          = {CSSB-BNITM-TG},
      ddc          = {610},
      cid          = {I:(DE-H253)CSSB-BNITM-TG-20210520},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project
                      G:(GEPRIS)240245660 - SFB 1129: Integrative Analyse der
                      Replikation und Ausbreitung pathogener Erreger (240245660) /
                      DFG project G:(GEPRIS)422048538 - SPP 2225:
                      Wirtszellaustritt intrazellulärer Pathogene (422048538)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)240245660 /
                      G:(GEPRIS)422048538},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39693377},
      UT           = {WOS:001380215900004},
      doi          = {10.1371/journal.ppat.1012788},
      url          = {https://bib-pubdb1.desy.de/record/619942},
}