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@INPROCEEDINGS{Grebinyk:619268,
      author       = {Grebinyk, Anna and Aminzadeh Gohari, Sepideh and Tarakci,
                      Elif and Komar, Yuliia and Stephan, Frank and Aftab, Namra
                      and Amirkhanyan, Zohrab and Boonpornprasert, Prach and
                      Borchert, Paul and Dmytriiev, Dmytro and Fisch, Anne-Sophie
                      and Frohme, Marcus and Good, James and Gross, Matthias and
                      Hoffmann, Andreas and Kalantaryan, Davit and Krasilnikov,
                      Mikhail and Li, Xiangkun and Lotfi, Zahra and Oppelt, Anne
                      and Radivoievych, Aleksandar and Richard, Christopher and
                      Riemer, Felix and Vashchenko, Grygorii and Villani, Daniel
                      and Worm, Steven and Zeeshan, Sumaira and Zhang, Xiaoyang},
      title        = {{ROS} generation and cellular response under irradiation
                      with the {FLASHLAB}@{PITZ} electron beam at ultra-high and
                      low dose rates},
      reportid     = {PUBDB-2024-07516},
      year         = {2024},
      abstract     = {Background and Aims: $R\&D$ platform FLASHlab@PITZ (Photo
                      Injector Test facility at DESY in Zeuthen) collaborates with
                      Technical University of Applied Sciences Wildau to provide
                      radiation oncology community with an in vitro and in vivo
                      infrastructure for systematic investigation of electron
                      irradiation at an extremely wide dose rates from
                      conventional (CONV) up to 1014 Gy/s. The initial biochemical
                      in vitro effects of the irradiation at FLASHlab@PITZ will be
                      presented in this contribution.Methods: Irradiation was
                      performed at startup FLASH-beamline using an 18 MeV PITZ
                      electron beam with doses ≤150Gy and dose rates of 0.05Gy/s
                      and 1.5-3.3x105Gy/s (UHDR). Hydrogen peroxide (H2O2)
                      production was estimated with AmplexRed at 4 and $1\%$ O2.
                      Survival, prooxidant and proapoptotic effects in human lung
                      carcinoma A549 and fibroblast HEL299 cells at $21\%$ O2were
                      evaluated using clonogenic,
                      2’,7’-Dichlorfluoreszeindiacetat and Caspase-Glo®3/7
                      assays, respectively. X-ray irradiation of cells was done at
                      0.015Gy/s with the 200keV X-ray generator
                      Comet-Yxlon-Maxishot.Results: H2O2 generation was hindered
                      with [O2] drop, while the lower H2O2 yield at UHDR compared
                      to CONV was consistent along all investigated [O2].
                      Decreased A549 and HEL299 cell survival was similar for CONV
                      and UHDR electron irradiation, following the effects of CONV
                      Xray irradiation. The intracellular ROS generation was
                      6-folds higher after CONV irradiation of HEL299 cells as
                      compared to UHDR. HEL299 cells were characterized with
                      gradual increase of the caspase 3/7 activity to $159±5\%$
                      after CONV with electron and X-rays, while no change was
                      detected after UHDR electron irradiation.Conclusions: CONV
                      irradiation had prooxidant and proapoptotic effect on
                      HEL299, but not on A549 cells. The increase of dose rate to
                      UHDR negated those effects, that pointed on its sparing
                      capability. However, CONV and UHDR irradiation had similar
                      effect on both cell survival, evidencing complex pattern of
                      cellular response and highlighting a need of in vivo
                      studies. FLASHlab@PITZ animal laboratory is in the final
                      preparation phase.},
      month         = {Dec},
      date          = {2024-12-04},
      organization  = {FLASH RADIOTHERAPY AND PARTICLE
                       THERAPY CONFERENCE, Rome (Italy), 4 Dec
                       2024 - 7 Dec 2024},
      cin          = {$Z_PITZ$ / $Z_DET$},
      cid          = {$I:(DE-H253)Z_PITZ-20210408$ / $I:(DE-H253)Z_DET-20201126$},
      pnm          = {621 - Accelerator Research and Development (POF4-621)},
      pid          = {G:(DE-HGF)POF4-621},
      experiment   = {EXP:(DE-H253)PITZ-20150101},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://bib-pubdb1.desy.de/record/619268},
}