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000619268 1001_ $$0P:(DE-H253)PIP1103334$$aGrebinyk, Anna$$b0
000619268 1112_ $$aFLASH RADIOTHERAPY AND PARTICLE THERAPY CONFERENCE$$cRome$$d2024-12-04 - 2024-12-07$$gFRPT 2024$$wItaly
000619268 245__ $$aROS generation and cellular response under irradiation with the FLASHLAB@PITZ electron beam at ultra-high and low dose rates
000619268 260__ $$c2024
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000619268 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1736177891_505764
000619268 520__ $$aBackground and Aims: R&D platform FLASHlab@PITZ (Photo Injector Test facility at DESY in Zeuthen) collaborates with Technical University of Applied Sciences Wildau to provide radiation oncology community with an in vitro and in vivo infrastructure for systematic investigation of electron irradiation at an extremely wide dose rates from conventional (CONV) up to 1014 Gy/s. The initial biochemical in vitro effects of the irradiation at FLASHlab@PITZ will be presented in this contribution.Methods: Irradiation was performed at startup FLASH-beamline using an 18 MeV PITZ electron beam with doses ≤150Gy and dose rates of 0.05Gy/s and 1.5-3.3x105Gy/s (UHDR). Hydrogen peroxide (H2O2) production was estimated with AmplexRed at 4 and 1% O2. Survival, prooxidant and proapoptotic effects in human lung carcinoma A549 and fibroblast HEL299 cells at 21% O2were evaluated using clonogenic, 2’,7’-Dichlorfluoreszeindiacetat and Caspase-Glo®3/7 assays, respectively. X-ray irradiation of cells was done at 0.015Gy/s with the 200keV X-ray generator Comet-Yxlon-Maxishot.Results: H2O2 generation was hindered with [O2] drop, while the lower H2O2 yield at UHDR compared to CONV was consistent along all investigated [O2]. Decreased A549 and HEL299 cell survival was similar for CONV and UHDR electron irradiation, following the effects of CONV Xray irradiation. The intracellular ROS generation was 6-folds higher after CONV irradiation of HEL299 cells as compared to UHDR. HEL299 cells were characterized with gradual increase of the caspase 3/7 activity to 159±5% after CONV with electron and X-rays, while no change was detected after UHDR electron irradiation.Conclusions: CONV irradiation had prooxidant and proapoptotic effect on HEL299, but not on A549 cells. The increase of dose rate to UHDR negated those effects, that pointed on its sparing capability. However, CONV and UHDR irradiation had similar effect on both cell survival, evidencing complex pattern of cellular response and highlighting a need of in vivo studies. FLASHlab@PITZ animal laboratory is in the final preparation phase.
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000619268 7001_ $$0P:(DE-H253)PIP1109705$$aAminzadeh Gohari, Sepideh$$b1$$eCorresponding author$$udesy
000619268 7001_ $$0P:(DE-H253)PIP1108811$$aTarakci, Elif$$b2
000619268 7001_ $$0P:(DE-H253)PIP1107003$$aKomar, Yuliia$$b3
000619268 7001_ $$0P:(DE-H253)PIP1004143$$aStephan, Frank$$b4
000619268 7001_ $$0P:(DE-H253)PIP1086967$$aAftab, Namra$$b5
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000619268 7001_ $$0P:(DE-HGF)0$$aBorchert, Paul$$b8
000619268 7001_ $$0P:(DE-H253)PIP1103583$$aDmytriiev, Dmytro$$b9
000619268 7001_ $$0P:(DE-HGF)0$$aFisch, Anne-Sophie$$b10
000619268 7001_ $$0P:(DE-H253)PIP1097695$$aFrohme, Marcus$$b11
000619268 7001_ $$0P:(DE-H253)PIP1014737$$aGood, James$$b12
000619268 7001_ $$0P:(DE-H253)PIP1013620$$aGross, Matthias$$b13
000619268 7001_ $$0P:(DE-H253)PIP1088516$$aHoffmann, Andreas$$b14
000619268 7001_ $$0P:(DE-H253)PIP1015089$$aKalantaryan, Davit$$b15
000619268 7001_ $$0P:(DE-H253)PIP1004128$$aKrasilnikov, Mikhail$$b16
000619268 7001_ $$0P:(DE-H253)PIP1081485$$aLi, Xiangkun$$b17
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000619268 7001_ $$0P:(DE-H253)PIP1011785$$aOppelt, Anne$$b19
000619268 7001_ $$0P:(DE-HGF)0$$aRadivoievych, Aleksandar$$b20
000619268 7001_ $$0P:(DE-H253)PIP1098846$$aRichard, Christopher$$b21
000619268 7001_ $$0P:(DE-H253)PIP1020058$$aRiemer, Felix$$b22
000619268 7001_ $$0P:(DE-H253)PIP1007520$$aVashchenko, Grygorii$$b23
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000619268 7001_ $$0P:(DE-H253)PIP1089976$$aWorm, Steven$$b25
000619268 7001_ $$0P:(DE-H253)PIP1106144$$aZeeshan, Sumaira$$b26
000619268 7001_ $$0P:(DE-H253)PIP1108379$$aZhang, Xiaoyang$$b27
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