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| 001 | 618994 | ||
| 005 | 20250723173021.0 | ||
| 024 | 7 | _ | |a 10.1021/acsnano.4c07701 |2 doi |
| 024 | 7 | _ | |a 1936-0851 |2 ISSN |
| 024 | 7 | _ | |a 1936-086X |2 ISSN |
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| 037 | _ | _ | |a PUBDB-2024-07304 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |a Mezgec, Klemen |0 P:(DE-H253)PIP1105027 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains |
| 260 | _ | _ | |a Washington, DC |c 2024 |b Soc. |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1734691692_2229497 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Modular protein engineering is a powerful approach for fabricating high-molecular-weight assemblies and biomaterials with nanoscale precision. Herein, we address the challenge of designing an extended nanoscale filamentous architecture inspired by the central rod domain of human dystrophin, which protects sarcolemma during muscle contraction and consists of spectrin repeats composed of three-helical bundles. A module of three tandem spectrin repeats was used as a rigid building block self-assembling via coiled-coil (CC) dimer-forming peptides. CC peptides were precisely integrated to maintain the spectrin α-helix continuity in an appropriate frame to form extended nanorods. An orthogonal set of customizable CC heterodimers was harnessed for modular rigid domain association, which could be additionally regulated by metal ions and chelators. We achieved a robust assembly of rigid rods several micrometers in length, determined by atomic force microscopy and negative stain transmission electron microscopy. Furthermore, these rigid rods can serve as a scaffold for the decoration of diverse proteins or biologically active peptides along their length with adjustable spacing up to tens of nanometers, as confirmed by the DNA-PAINT super-resolution microscopy. This demonstrates the potential of modular bottom-up protein engineering and tunable CCs for the fabrication of functionalized protein biomaterials. |
| 536 | _ | _ | |a 6G3 - PETRA III (DESY) (POF4-6G3) |0 G:(DE-HGF)POF4-6G3 |c POF4-6G3 |f POF IV |x 0 |
| 536 | _ | _ | |a CTGCT - Centre of Excellence for the Technologies of Gene and Cell Therapy (101059842) |0 G:(EU-Grant)101059842 |c 101059842 |f HORIZON-WIDERA-2022-ACCESS-01-two-stage |x 1 |
| 536 | _ | _ | |a LoopOfFun - Closed-loop control of fungal materials (101070817) |0 G:(EU-Grant)101070817 |c 101070817 |f HORIZON-EIC-2021-PATHFINDERCHALLENGES-01 |x 2 |
| 536 | _ | _ | |a VIROFIGHT - General-purpose virus-neutralizing engulfing shells with modular target-specificity (899619) |0 G:(EU-Grant)899619 |c 899619 |f H2020-FETOPEN-2018-2019-2020-01 |x 3 |
| 542 | _ | _ | |i 2024-10-11 |2 Crossref |u https://creativecommons.org/licenses/by/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: bib-pubdb1.desy.de |
| 693 | _ | _ | |a PETRA III |f PETRA Beamline P12 |1 EXP:(DE-H253)PETRAIII-20150101 |0 EXP:(DE-H253)P-P12-20150101 |6 EXP:(DE-H253)P-P12-20150101 |x 0 |
| 700 | 1 | _ | |a Snoj, Jaka |b 1 |
| 700 | 1 | _ | |a Ulčakar, Liza |b 2 |
| 700 | 1 | _ | |a Ljubetič, Ajasja |b 3 |
| 700 | 1 | _ | |a Tušek Žnidarič, Magda |b 4 |
| 700 | 1 | _ | |a Škarabot, Miha |0 0000-0001-5734-7839 |b 5 |
| 700 | 1 | _ | |a Jerala, Roman |b 6 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1021/acsnano.4c07701 |b American Chemical Society (ACS) |d 2024-10-11 |n 42 |p 28748-28763 |3 journal-article |2 Crossref |t ACS Nano |v 18 |y 2024 |x 1936-0851 |
| 773 | _ | _ | |a 10.1021/acsnano.4c07701 |g Vol. 18, no. 42, p. 28748 - 28763 |0 PERI:(DE-600)2383064-5 |n 42 |p 28748-28763 |t ACS nano |v 18 |y 2024 |x 1936-0851 |
| 856 | 4 | _ | |u https://pubs.acs.org/doi/10.1021/acsnano.4c07701 |
| 856 | 4 | _ | |u https://bib-pubdb1.desy.de/record/618994/files/Coupling%20of%20Spectrin%20Repeat%20Modules%20for%20the%20Assembly%20of%20Nanorods%20and%20Presentation%20of%20Protein%20Domains.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://bib-pubdb1.desy.de/record/618994/files/Coupling%20of%20Spectrin%20Repeat%20Modules%20for%20the%20Assembly%20of%20Nanorods%20and%20Presentation%20of%20Protein%20Domains.pdf?subformat=pdfa |x pdfa |y OpenAccess |
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| 910 | 1 | _ | |a European Molecular Biology Laboratory |0 I:(DE-588b)235011-7 |k EMBL |b 0 |6 P:(DE-H253)PIP1105027 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 0 |6 P:(DE-H253)PIP1105027 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Großgeräte: Materie |1 G:(DE-HGF)POF4-6G0 |0 G:(DE-HGF)POF4-6G3 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-600 |4 G:(DE-HGF)POF |v PETRA III (DESY) |x 0 |
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| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
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| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b ACS NANO : 2022 |d 2025-01-07 |
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