TY  - JOUR
AU  - Patel, Jaydeep
AU  - Round, Adam
AU  - de Wijn, Raphael
AU  - Vakili, Mohammad
AU  - Giovanetti, Gabriele
AU  - Melo, Diogo Filipe Monrroy Vilan e
AU  - E, Juncheng
AU  - Sikorski, Marcin
AU  - Koliyadu, Jayanth
AU  - Koua, Faisal H. M.
AU  - Sato, Tokushi
AU  - Mancuso, Adrian
AU  - Peele, Andrew
AU  - Abbey, Brian
TI  - Real-time analysis of liquid jet sample delivery stability for an X-ray free-electron laser using machine vision
JO  - Journal of applied crystallography
VL  - 57
IS  - 6
SN  - 1600-5767
CY  - Chester, UK
PB  - Wiley-Blackwell
M1  - PUBDB-2024-07164
SP  - 1859-1870
PY  - 2024
AB  - Automated evaluation of optical microscopy images of liquid jets, commonlyused for sample delivery at X-ray free-electron lasers (XFELs), enables real-time tracking of the jet position and liquid jet hit rates, defined here as theproportion of XFEL pulses intersecting with the liquid jet. This method utilizesmachine vision for preprocessing, feature extraction, segmentation and jetdetection as well as tracking to extract key physical characteristics (such as thejet angle) from optical microscopy images captured during experiments. Todetermine the effectiveness of these tools in monitoring jet stability andenhancing sample delivery efficiency, we conducted XFEL experiments withvarious sample compositions (pure water, buffer and buffer with crystals),nozzle designs and jetting conditions. We integrated our real-time analysisalgorithm into the Karabo control system at the European XFEL. The resultsindicate that the algorithm performs well in monitoring the jet angle andprovides a quantitative characterization of liquid jet stability through opticalimage analysis conducted during experiments
LB  - PUB:(DE-HGF)16
C6  - 39628876
UR  - <Go to ISI:>//WOS:001387959000015
DO  - DOI:10.1107/S1600576724009853
UR  - https://bib-pubdb1.desy.de/record/618810
ER  -