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@ARTICLE{Reinke:617263,
author = {Reinke, Patrick Y. A. and Heiringhoff, Robin S. and Reindl,
Theresia and Baker, Karen and Taft, Manuel H. and Meents,
Alke and Mulvihill, Daniel P. and Davies, Owen R. and
Fedorov, Roman and Zahn, Michael and Manstein, Dietmar J.},
title = {{C}rystal structures of cables formed by the acetylated and
unacetylated forms of the {S}chizosaccharomyces pombe
tropomyosin orthologue {T}pm{C}dc8},
journal = {Biologist},
volume = {300},
number = {12},
issn = {0021-9258},
address = {Bethesda, MD},
publisher = {American Soc. for Biochemistry and Molecular Biology},
reportid = {PUBDB-2024-06660},
pages = {107925},
year = {2024},
note = {ISSN 0021-9258 not unique: **2 hits**. erman Research
Foundation (DFG), Grant FE1510/2-1, and the Cluster of
Excellence RESIST (Resolving InfectionSusceptibility; EXC
2155), project ID: 39087428},
abstract = {Cables formed by head-to-tail polymerization of
tropomyosin, localized along the length of sarcomeric and
cytoskeletal actin filaments, play a key role in regulating
a wide range of motile and contractile processes. The
stability of tropomyosin cables, their interaction with
actin filaments and the functional properties of the
resulting co-filaments are thought to be affected by
N-terminal acetylation of tropomyosin. Here, we present
high-resolution structures of cables formed by acetylated
and unacetylated Schizosaccharomyces pombe tropomyosin
orthologue TpmCdc8. The crystal structures represent
different types of cables, each consisting of TpmCdc8
homodimers in a different conformation. The structures show
how the interactions of the residues in the overlap junction
contribute to cable formation and how local structural
perturbations affect the conformational dynamics of the
protein and its ability to transmit allosteric signals. In
particular, N-terminal acetylation increases the helicity of
the adjacent region, which leads to a local reduction in
conformational dynamics and consequently to less fraying of
the N-terminal region. This creates a more consistent
complementary surface facilitating the formation of specific
interactions across the overlap junction.},
cin = {FS-CFEL-1-BMX / FS-CFEL-1},
ddc = {610},
cid = {I:(DE-H253)FS-CFEL-1-BMX-20210408 /
I:(DE-H253)FS-CFEL-1-20120731},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)
/ DFG project G:(GEPRIS)390874280 - EXC 2155: RESIST -
Resolving Infection Susceptibility (390874280)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3 /
G:(GEPRIS)390874280},
experiment = {EXP:(DE-H253)P-P13-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39461476},
UT = {WOS:001366933400001},
doi = {10.1016/j.jbc.2024.107925},
url = {https://bib-pubdb1.desy.de/record/617263},
}