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@ARTICLE{Reichmann:616197,
      author       = {Reichmann, Jakob and Franz, Jonas and Eckermann, Marina and
                      Schulz, Katja and Mollenhauer, Brit and Stadelmann,
                      Christine and Salditt, Tim},
      title        = {3{D} imaging of neuronal inclusions and protein aggregates
                      in human neurodegeneration by multiscale {X}-ray
                      phase-contrast tomography},
      reportid     = {PUBDB-2024-06357},
      year         = {2024},
      abstract     = {This study leverages X-ray phase-contrast tomography for
                      detailed analysis of neurodegenerative diseases focusing on
                      the 3D visualization and quantification of neuropathological
                      features within fixed human postmortem tissue. X-ray
                      phase-contrast tomography with synchrotron radiation offers
                      micrometer and even sub-micron resolution, enabling us to
                      examine intra- and extraneuronal aggregates and inclusions
                      such as Lewy bodies, granulovacuolar degeneration, Hirano
                      bodies, neurofibrillary tangles, β-amyloid plaques and
                      vascular amyloid deposits in three dimensions (3D). In the
                      reconstructions, we identified the highest electron
                      densities in Hirano bodies and Lewy bodies while
                      neurofibrillary tangles exhibit only slight alterations in
                      X-ray phase-contrast tomography contrast. Using cutting edge
                      high-resolution X-ray synchrotron beamlines we are now able
                      to even detect subcellular differences of electron densities
                      found in granulovacuolar degeneration. Small scale
                      inhomogeneities of the electron density are also detected in
                      Lewy bodies potentially relating to inclusions of
                      organelles. Additionally, we reveal a peculiar 3D geometry
                      of Hirano bodies and demonstrate the co-occurence with
                      granulovacuolar degeneration in the same neuron. Utilizing
                      X-ray phase-contrast tomography in a complementary fashion
                      to traditional technologies, a quantitative, systematic and
                      disease-overarching understanding of inclusions and
                      aggregates in neurodegeneration can be achieved.},
      cin          = {DOOR ; HAS-User},
      cid          = {I:(DE-H253)HAS-User-20120731},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / SFB 1456 A03 -
                      Dimensionalitätsreduktion und Regression im
                      Wasserstein-Raum für quantitative 3D-Histologie (A03)
                      (456837373) / DFG project G:(GEPRIS)390729940 - EXC 2067:
                      Multiscale Bioimaging: Von molekularen Maschinen zu
                      Netzwerken erregbarer Zellen (390729940)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(GEPRIS)456837373 /
                      G:(GEPRIS)390729940},
      experiment   = {EXP:(DE-H253)P-P10-20150101},
      typ          = {PUB:(DE-HGF)25},
      doi          = {10.1101/2024.03.26.24304193},
      url          = {https://bib-pubdb1.desy.de/record/616197},
}