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@ARTICLE{Albers:614277,
      author       = {Albers, Suki and Beckert, Bertrand and Matthies, Marco C.
                      and Mandava, Chandra Sekhar and Schuster, Raphael and
                      Seuring, Carolin and Riedner, Maria and Sanyal, Suparna and
                      Torda, Andrew E. and Wilson, Daniel N. and Ignatova, Zoya},
      title        = {{R}epurposing t{RNA}s for nonsense suppression},
      journal      = {Nature Communications},
      volume       = {12},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {PUBDB-2024-05817},
      pages        = {3850},
      year         = {2021},
      note         = {This research was supported by grants from the Deutsche
                      Forschungsgemeinschaft IG73/14-2 (to Z.I.) and WI2381/6-1
                      (to D.N.W.)},
      abstract     = {Three stop codons (UAA, UAG and UGA) terminate protein
                      synthesis and are almost exclusively recognized by release
                      factors. Here, we design de novo transfer RNAs (tRNAs) that
                      efficiently decode UGA stop codons in Escherichia coli. The
                      tRNA designs harness various functionally conserved aspects
                      of sense-codon decoding tRNAs. Optimization within the
                      TΨC-stem to stabilize binding to the elongation factor,
                      displays the most potent effect in enhancing suppression
                      activity. We determine the structure of the ribosome in a
                      complex with the designed tRNA bound to a UGA stop codon in
                      the A site at 2.9 Å resolution. In the context of the
                      suppressor tRNA, the conformation of the UGA codon resembles
                      that of a sense-codon rather than when canonical translation
                      termination release factors are bound, suggesting
                      conformational flexibility of the stop codons dependent on
                      the nature of the A-site ligand. The systematic analysis,
                      combined with structural insights, provides a rationale for
                      targeted repurposing of tRNAs to correct devastating
                      nonsense mutations that introduce a premature stop codon.},
      cin          = {CSSB-CF-CRYO},
      ddc          = {500},
      cid          = {I:(DE-H253)CSSB-CF-CRYO-20210520},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34158503},
      UT           = {WOS:000669043500017},
      doi          = {10.1038/s41467-021-24076-x},
      url          = {https://bib-pubdb1.desy.de/record/614277},
}