000614277 001__ 614277
000614277 005__ 20250929155758.0
000614277 0247_ $$2doi$$a10.1038/s41467-021-24076-x
000614277 0247_ $$2datacite_doi$$a10.3204/PUBDB-2024-05817
000614277 0247_ $$2altmetric$$aaltmetric:108073672
000614277 0247_ $$2pmid$$apmid:34158503
000614277 0247_ $$2WOS$$aWOS:000669043500017
000614277 0247_ $$2openalex$$aopenalex:W3177145028
000614277 037__ $$aPUBDB-2024-05817
000614277 041__ $$aEnglish
000614277 082__ $$a500
000614277 1001_ $$aAlbers, Suki$$b0
000614277 245__ $$aRepurposing tRNAs for nonsense suppression
000614277 260__ $$a[London]$$bNature Publishing Group UK$$c2021
000614277 3367_ $$2DRIVER$$aarticle
000614277 3367_ $$2DataCite$$aOutput Types/Journal article
000614277 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1729084489_2584836
000614277 3367_ $$2BibTeX$$aARTICLE
000614277 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000614277 3367_ $$00$$2EndNote$$aJournal Article
000614277 500__ $$aThis research was supported by grants from the Deutsche Forschungsgemeinschaft IG73/14-2 (to Z.I.) and WI2381/6-1 (to D.N.W.) 
000614277 520__ $$aThree stop codons (UAA, UAG and UGA) terminate protein synthesis and are almost exclusively recognized by release factors. Here, we design de novo transfer RNAs (tRNAs) that efficiently decode UGA stop codons in Escherichia coli. The tRNA designs harness various functionally conserved aspects of sense-codon decoding tRNAs. Optimization within the TΨC-stem to stabilize binding to the elongation factor, displays the most potent effect in enhancing suppression activity. We determine the structure of the ribosome in a complex with the designed tRNA bound to a UGA stop codon in the A site at 2.9 Å resolution. In the context of the suppressor tRNA, the conformation of the UGA codon resembles that of a sense-codon rather than when canonical translation termination release factors are bound, suggesting conformational flexibility of the stop codons dependent on the nature of the A-site ligand. The systematic analysis, combined with structural insights, provides a rationale for targeted repurposing of tRNAs to correct devastating nonsense mutations that introduce a premature stop codon. 
000614277 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0
000614277 588__ $$aDataset connected to DataCite
000614277 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0
000614277 7001_ $$aBeckert, Bertrand$$b1
000614277 7001_ $$aMatthies, Marco C.$$b2
000614277 7001_ $$aMandava, Chandra Sekhar$$b3
000614277 7001_ $$aSchuster, Raphael$$b4
000614277 7001_ $$0P:(DE-H253)PIP1020921$$aSeuring, Carolin$$b5
000614277 7001_ $$aRiedner, Maria$$b6
000614277 7001_ $$00000-0002-7124-792X$$aSanyal, Suparna$$b7
000614277 7001_ $$00000-0002-6076-709X$$aTorda, Andrew E.$$b8
000614277 7001_ $$00000-0003-3816-3828$$aWilson, Daniel N.$$b9
000614277 7001_ $$00000-0002-9478-8825$$aIgnatova, Zoya$$b10$$eCorresponding author
000614277 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-021-24076-x$$gVol. 12, no. 1, p. 3850$$n1$$p3850$$tNature Communications$$v12$$x2041-1723$$y2021
000614277 8564_ $$uhttps://bib-pubdb1.desy.de/record/614277/files/s41467-021-24076-x.pdf$$yOpenAccess
000614277 8564_ $$uhttps://bib-pubdb1.desy.de/record/614277/files/s41467-021-24076-x.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000614277 909CO $$ooai:bib-pubdb1.desy.de:614277$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000614277 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1020921$$aCentre for Free-Electron Laser Science$$b5$$kCFEL
000614277 9101_ $$0I:(DE-H253)_CSSB-20140311$$6P:(DE-H253)PIP1020921$$aCentre for Structural Systems Biology$$b5$$kCSSB
000614277 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1020921$$aEuropean XFEL$$b5$$kXFEL.EU
000614277 9131_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000614277 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-05-02T09:09:09Z
000614277 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT COMMUN : 2022$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-05-02T09:09:09Z
000614277 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000614277 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-08-29
000614277 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000614277 915__ $$0StatID:(DE-HGF)9915$$2StatID$$aIF >= 15$$bNAT COMMUN : 2022$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2023-05-02T09:09:09Z
000614277 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-08-29
000614277 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2023-08-29
000614277 9201_ $$0I:(DE-H253)CSSB-CF-CRYO-20210520$$kCSSB-CF-CRYO$$lCSSB-CF-CRYO$$x0
000614277 980__ $$ajournal
000614277 980__ $$aVDB
000614277 980__ $$aUNRESTRICTED
000614277 980__ $$aI:(DE-H253)CSSB-CF-CRYO-20210520
000614277 9801_ $$aFullTexts