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@ARTICLE{Kim:614236,
      author       = {Kim, Dojin and Bossi, Mariano L. and Belov, Vladimir N. and
                      Hell, Stefan W.},
      title        = {{S}upramolecular {C}omplex of {C}ucurbit[7]uril with
                      {D}iketopyrrolopyrole {D}ye: {F}luorescence {B}oost,
                      {B}iolabeling and {O}ptical {M}icroscopy},
      journal      = {Angewandte Chemie / International edition},
      volume       = {63},
      number       = {36},
      issn         = {1433-7851},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {PUBDB-2024-05776},
      pages        = {e202410217},
      year         = {2024},
      abstract     = {New photostable and bright supramolecular complexes based
                      on cucurbit[7]uril (CB7) host and diketopyrrolopyrole (DPP)
                      guest dyes having two positively charged
                      4-(trimethylammonio)phenyl groups were prepared and
                      characterized. The dye core displays large Stokes shift (in
                      H2O, abs./emission max. 480/550 nm; ϵ~19 000, τfl>4
                      ns), strong binding with the host (~560 nM Kd) and a
                      linker affording fluorescence detection of bioconjugates
                      with antibody and nanobody. Combination of
                      protein-functionalized DPP dye with CB7 improves
                      photostability and affords up to 12-fold emission gain.
                      Two-color confocal and stimulated emission depletion (STED)
                      microscopy with 595 nm or 655 nm STED depletion lasers
                      shows that the presence of CB7 not only leads to improved
                      brightness and image quality, but also results in DPP
                      becoming cell-permeable.},
      cin          = {CSSB-CF-SPC},
      ddc          = {540},
      cid          = {I:(DE-H253)CSSB-CF-SPC-20210520},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38881490},
      UT           = {WOS:001282939000001},
      doi          = {10.1002/anie.202410217},
      url          = {https://bib-pubdb1.desy.de/record/614236},
}