TY  - JOUR
AU  - Kim, Dojin
AU  - Bossi, Mariano L.
AU  - Belov, Vladimir N.
AU  - Hell, Stefan W.
TI  - Supramolecular Complex of Cucurbit[7]uril with Diketopyrrolopyrole Dye: Fluorescence Boost, Biolabeling and Optical Microscopy
JO  - Angewandte Chemie / International edition
VL  - 63
IS  - 36
SN  - 1433-7851
CY  - Weinheim
PB  - Wiley-VCH
M1  - PUBDB-2024-05776
SP  - e202410217
PY  - 2024
AB  - New photostable and bright supramolecular complexes based on cucurbit[7]uril (CB7) host and diketopyrrolopyrole (DPP) guest dyes having two positively charged 4-(trimethylammonio)phenyl groups were prepared and characterized. The dye core displays large Stokes shift (in H2O, abs./emission max. 480/550 nm; ϵ 19 000, τfl>4 ns), strong binding with the host ( 560 nM Kd) and a linker affording fluorescence detection of bioconjugates with antibody and nanobody. Combination of protein-functionalized DPP dye with CB7 improves photostability and affords up to 12-fold emission gain. Two-color confocal and stimulated emission depletion (STED) microscopy with 595 nm or 655 nm STED depletion lasers shows that the presence of CB7 not only leads to improved brightness and image quality, but also results in DPP becoming cell-permeable. 
LB  - PUB:(DE-HGF)16
C6  - pmid:38881490
UR  - <Go to ISI:>//WOS:001282939000001
DO  - DOI:10.1002/anie.202410217
UR  - https://bib-pubdb1.desy.de/record/614236
ER  -