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@ARTICLE{Wang:614231,
      author       = {Wang, Mengying and Thünauer, Roland and Schubert, Robin
                      and Gevorgyan, Susanna and Lorenzen, Kristina and Brognaro,
                      Hévila and Betzel, Christian},
      title        = {{F}ormation kinetics and physicochemical properties of
                      mesoscopic {A}lpha-{S}ynuclein assemblies modulated by
                      sodium chloride and a distinct pulsed electric field},
      journal      = {Soft matter},
      volume       = {19},
      number       = {7},
      issn         = {1744-683X},
      address      = {London},
      publisher    = {Royal Soc. of Chemistry},
      reportid     = {PUBDB-2024-05771},
      pages        = {1363 - 1372},
      year         = {2023},
      abstract     = {Alpha-Synuclein (ASN), a presynaptic protein, has been
                      widely reported to form amyloid-rich hydrogel clusters
                      through liquid–liquid phase separation (LLPS) and
                      liquid-to-solid transition. However, in-depth investigations
                      about the parameters that influence the assembling kinetics,
                      structures, and physicochemical properties of intermediate
                      ASN assemblies are still missing. Therefore, we monitored
                      for the first time the assembling and ordering kinetics of
                      ASN by polarized/depolarized light scattering (DLS/DDLS)
                      under the effect of ionic strength and a pulsed electric
                      field (EF), followed by characterizing the resultant ASN
                      assemblies applying thermostability assays,
                      fluorescence/autofluorescence assays, and TEM. The
                      underlying molecular mechanism was discussed based on
                      experimental evidence. Results showed that in the presence
                      of 150–250 mM NaCl, monomeric ASN is highly soluble in a
                      temperature range of 20–70 °C and could form dissoluble
                      liquid dense clusters via LLPS in crowded environments,
                      while the ionic strength of 50 mM NaCl could trigger
                      conformational changes and attractive diffusion interactions
                      of ASN monomers towards the formation of mesoscopic
                      assemblies with ordered internal structures and high
                      thermostabilities. We discovered that pulsed EFs and ionic
                      strength can modulate effectively the thermostability and
                      autofluorescence effect of mesoscopic ASN assemblies by
                      tuning the molecular interaction and arrangement.
                      Remarkably, a specie of thermostable ASN assemblies showing
                      a maximum autofluorescence emission at approx. 700 nm was
                      synthesized applying 250 mM NaCl and the distinct pulsed EF,
                      which could be attributed to the increase of β-sheet
                      structures and hydrogen-bond networks within ASN assemblies.
                      In summary, the presented data provide novel insights for
                      modulating the growth kinetics, structures, and
                      physicochemical properties of bio-macromolecular mesoscopic
                      assemblies.},
      cin          = {CSSB-CF-ALFM},
      ddc          = {530},
      cid          = {I:(DE-H253)CSSB-CF-ALFM-20210629},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project
                      G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of
                      Matter (390715994) / DFG project G:(GEPRIS)194651731 - EXC
                      1074: Hamburger Zentrum für ultraschnelle Beobachtung
                      (CUI): Struktur, Dynamik und Kontrolle von Materie auf
                      atomarer Skala (194651731)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)390715994 /
                      G:(GEPRIS)194651731},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36723049},
      UT           = {WOS:000920904800001},
      doi          = {10.1039/D2SM01615J},
      url          = {https://bib-pubdb1.desy.de/record/614231},
}