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@INBOOK{Custodio:614015,
      author       = {Custodio, Tania and Guédez, Gabriela and Loew, Christian},
      title        = {{T}ransient {C}o-expression of {M}embrane {P}rotein
                      {C}omplexes in {M}ammalian {C}ells},
      volume       = {2810},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {PUBDB-2024-05716},
      isbn         = {978-1-0716-3877-4 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {11 - 28},
      year         = {2024},
      comment      = {Recombinant Protein Expression in Mammalian Cells / Hacker,
                      David L. (Editor) ; New York, NY : Springer US, 2024,
                      Chapter 2 ; ISSN: 1064-3745=1940-6029 ; ISBN:
                      978-1-0716-3877-4=978-1-0716-3878-1 ;
                      doi:10.1007/978-1-0716-3878-1},
      booktitle     = {Recombinant Protein Expression in
                       Mammalian Cells / Hacker, David L.
                       (Editor) ; New York, NY : Springer US,
                       2024, Chapter 2 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-3877-4=978-1-0716-3878-1 ;
                       doi:10.1007/978-1-0716-3878-1},
      abstract     = {Membrane proteins are essential components of biological
                      membranes with key roles in cellular processes such as
                      nutrient transport, cell communication, signaling, or energy
                      conversion. Due to their crucial functions, membrane
                      proteins and their complexes are often targets for
                      therapeutic interventions. Expression and purification of
                      membrane proteins are often a bottleneck to yield sufficient
                      material for structural studies and further downstream
                      characterization. Taking advantage of the Expi293 expression
                      system for the production of eukaryotic proteins, we present
                      a very efficient and fast protocol for the co-expression of
                      a membrane complex. Here, we use transient transfection to
                      co-express the membrane transporter PHT1 with its adaptor
                      protein TASL. To allow the simultaneous screening of
                      different proteins, constructs, or interaction partners, we
                      make use of the Twin-Strep magnetic system. The protocol can
                      be applied for small-scale screening of any membrane protein
                      alone or co-expressed with interacting partners followed by
                      large-scale production and purification of a potential
                      membrane protein complex.},
      cin          = {CSSB-EMBL-CL},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-EMBL-CL-20210806},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)7},
      doi          = {10.1007/978-1-0716-3878-1_2},
      url          = {https://bib-pubdb1.desy.de/record/614015},
}