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000614015 0247_ $$2doi$$a10.1007/978-1-0716-3878-1_2
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000614015 0247_ $$2ISSN$$a1940-6029
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000614015 041__ $$aEnglish
000614015 082__ $$a570
000614015 1001_ $$0P:(DE-H253)PIP1091456$$aCustodio, Tania$$b0$$eCorresponding author
000614015 245__ $$aTransient Co-expression of Membrane Protein Complexes in Mammalian Cells
000614015 260__ $$aNew York, NY$$bSpringer US$$c2024
000614015 29510 $$aRecombinant Protein Expression in Mammalian Cells / Hacker, David L. (Editor) ; New York, NY : Springer US, 2024, Chapter 2 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-3877-4=978-1-0716-3878-1 ; doi:10.1007/978-1-0716-3878-1
000614015 300__ $$a11 - 28
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000614015 4900_ $$aMethods in Molecular Biology$$v2810
000614015 520__ $$aMembrane proteins are essential components of biological membranes with key roles in cellular processes such as nutrient transport, cell communication, signaling, or energy conversion. Due to their crucial functions, membrane proteins and their complexes are often targets for therapeutic interventions. Expression and purification of membrane proteins are often a bottleneck to yield sufficient material for structural studies and further downstream characterization. Taking advantage of the Expi293 expression system for the production of eukaryotic proteins, we present a very efficient and fast protocol for the co-expression of a membrane complex. Here, we use transient transfection to co-express the membrane transporter PHT1 with its adaptor protein TASL. To allow the simultaneous screening of different proteins, constructs, or interaction partners, we make use of the Twin-Strep magnetic system. The protocol can be applied for small-scale screening of any membrane protein alone or co-expressed with interacting partners followed by large-scale production and purification of a potential membrane protein complex. 
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000614015 7001_ $$0P:(DE-H253)PIP1093804$$aGuédez, Gabriela$$b1
000614015 7001_ $$0P:(DE-H253)PIP1030211$$aLoew, Christian$$b2$$eCorresponding author
000614015 773__ $$a10.1007/978-1-0716-3878-1_2
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