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@ARTICLE{Mortensen:613926,
author = {Mortensen, Sofia and Kuncova, Stanislava and Lormand,
Justin David and Myers, Tanner and Kim, Soo-Kyoung and Lee,
Vincent and Winkler, Wade and Sondermann, Holger},
title = {{S}tructural and bioinformatics analyses identify
deoxydinucleotide-specific nucleasesand their association
with genomic islands in {G}ram-positive bacteria},
journal = {Nucleic acids research},
volume = {53},
number = {1},
issn = {0305-1048},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {PUBDB-2024-05705},
pages = {gkae1235},
year = {2025},
note = {ISSN 1362-4962 not unique: **2 hits**.},
abstract = {Dinucleases of the DEDD superfamily, such as
oligoribonuclease, Rexo2, and nanoRNaseC, catalyze the
essential final step of RNA degradation, the conversion of
di- tomononucleotides. The active sites of these enzymes are
optimized for substrates that are twonucleotides long, and
do not discriminate between RNA and DNA. Here, we identified
a novelDEDD subfamily, members of which function as
dedicated deoxydinucleases (diDNases) thatspecifically
hydrolyze single-stranded DNA dinucleotides in a
sequence-independent manner.Crystal structures of
enzyme-substrate complexes reveal that specificity for DNA
stems froma combination of conserved structural elements
that exclude diribonucleotides as substrates.Consistently,
diDNases fail to complement the loss of enzymes that act on
diribonucleotides,indicating that these two groups of
enzymes support distinct cellular functions. The
genesencoding diDNases are found predominantly in genomic
islands of Actinomycetes andClostridia, which, together with
their association with phage-defense systems,
suggestpotential roles in bacterial immunity.},
cin = {CSSB-DESY-HS / FS DOOR-User / CSSB-CF-SPC},
ddc = {570},
cid = {I:(DE-H253)CSSB-DESY-HS-20210521 /
$I:(DE-H253)FS_DOOR-User-20241023$ /
I:(DE-H253)CSSB-CF-SPC-20210520},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P11-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39778863},
doi = {10.1093/nar/gkae1235},
url = {https://bib-pubdb1.desy.de/record/613926},
}