TY  - JOUR
AU  - Hover, Samantha
AU  - Charlton, Frank W.
AU  - Hellert, Jan
AU  - Swanson, Jessica J.
AU  - Mankouri, Jamel
AU  - Barr, John N.
AU  - Fontana, Juan
TI  - Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K+ during entry
JO  - Nature Communications
VL  - 14
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - PUBDB-2024-05666
SP  - 5885
PY  - 2023
AB  - Following endocytosis, enveloped viruses employ the changing environmentof maturing endosomes as cues to promote endosomal escape, a processoften mediated by viral glycoproteins. We previously showed that both high[K+] and low pH promote entry of Bunyamwera virus (BUNV), the prototypicalbunyavirus. Here, we use sub-tomogram averaging and AlphaFold, to generatea pseudo-atomic model of the whole BUNV glycoprotein envelope. Weunambiguously locate the Gc fusion domain and its chaperone Gn within thefloor domain of the spike. Furthermore, viral incubation at low pH and high[K+], reminiscent of endocytic conditions, results in a dramatic rearrangementof the BUNV envelope. Structural and biochemical assays indicate that pH 6.3/K+ in the absence of a target membrane elicits a fusion-capable triggeredintermediate state of BUNV GPs; but the same conditions induce fusion whentarget membranes are present. Taken together, we provide mechanisticunderstanding of the requirements for bunyavirus entry 
LB  - PUB:(DE-HGF)16
C6  - pmid:37735161
UR  - <Go to ISI:>//WOS:001075884500005
DO  - DOI:10.1038/s41467-023-41205-w
UR  - https://bib-pubdb1.desy.de/record/613887
ER  -