000613884 001__ 613884
000613884 005__ 20250715170925.0
000613884 0247_ $$2doi$$a10.1093/nar/gkae374
000613884 0247_ $$2ISSN$$a0305-1048
000613884 0247_ $$2ISSN$$a0261-3166
000613884 0247_ $$2ISSN$$a1362-4954
000613884 0247_ $$2ISSN$$a1362-4962
000613884 0247_ $$2ISSN$$a1746-8272
000613884 0247_ $$2datacite_doi$$a10.3204/PUBDB-2024-05663
000613884 0247_ $$2altmetric$$aaltmetric:163909166
000613884 0247_ $$2pmid$$apmid:38806233
000613884 0247_ $$2WOS$$aWOS:001233236800001
000613884 0247_ $$2openalex$$aopenalex:W4399148883
000613884 037__ $$aPUBDB-2024-05663
000613884 041__ $$aEnglish
000613884 082__ $$a540
000613884 1001_ $$aGustavsson, Emil$$b0
000613884 245__ $$aDynamics of the Herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-reading revealed by Cryo-EM
000613884 260__ $$aOxford$$bOxford Univ. Press$$c2024
000613884 3367_ $$2DRIVER$$aarticle
000613884 3367_ $$2DataCite$$aOutput Types/Journal article
000613884 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1729068097_2584733
000613884 3367_ $$2BibTeX$$aARTICLE
000613884 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000613884 3367_ $$00$$2EndNote$$aJournal Article
000613884 500__ $$aThe CryoEM Facility at CSSB is supported by the UHH and DFG [INST 152/772-1|152/774-1|152/775-1|152/776-1|152/777-1 FUGG].   
000613884 520__ $$aHerpes simplex virus 1 (HSV-1), a double-stranded DNA virus, replicates using seven essential proteins encoded by its genome. Among these, the UL30 DNA polymerase, complexed with the UL42 processivity factor, orchestrates leading and lagging strand replication of the 152 kb viral genome. UL30 polymerase is a prime target for antiviral therapy, and resistance to current drugs can arise in immunocompromised individuals. Using electron cryo-microscopy (cryo-EM), we unveil the dynamic changes of the UL30/UL42 complex with DNA in three distinct states. First, a pre-translocation state with an open fingers domain ready for nucleotide incorporation. Second, a halted elongation state where the fingers close, trapping dATP in the dNTP pocket. Third, a DNA-editing state involving significant conformational changes to allow DNA realignment for exonuclease activity. Additionally, the flexible UL30 C-terminal domain interacts with UL42, forming an extended positively charged surface binding to DNA, thereby enhancing processive synthesis. These findings highlight substantial structural shifts in the polymerase and its DNA interactions during replication, offering insights for future antiviral drug development. 
000613884 536__ $$0G:(DE-HGF)POF4-633$$a633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633)$$cPOF4-633$$fPOF IV$$x0
000613884 536__ $$0G:(GEPRIS)534044797$$aDFG project G:(GEPRIS)534044797 - Large Scale Data Facility 3 - Anteil Forschungsgroßgerät (LSDF3-FuGG) (534044797)$$c534044797$$x1
000613884 542__ $$2Crossref$$i2024-05-29$$uhttps://creativecommons.org/licenses/by/4.0/
000613884 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de
000613884 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0
000613884 7001_ $$0P:(DE-H253)PIP1023782$$aGrünewald, Kay$$b1$$udesy
000613884 7001_ $$0P:(DE-HGF)0$$aElias, Per$$b2
000613884 7001_ $$0P:(DE-HGF)0$$aHällberg, B Martin$$b3$$eCorresponding author
000613884 77318 $$2Crossref$$3journal-article$$a10.1093/nar/gkae374$$bOxford University Press (OUP)$$d2024-05-29$$n12$$p7292-7304$$tNucleic Acids Research$$v52$$x0305-1048$$y2024
000613884 773__ $$0PERI:(DE-600)2205588-5$$a10.1093/nar/gkae374$$gVol. 52, no. 12, p. 7292 - 7304$$n12$$p7292-7304$$tNucleic acids symposium series$$v52$$x0305-1048$$y2024
000613884 8564_ $$uhttps://bib-pubdb1.desy.de/record/613884/files/gkae374.pdf$$yOpenAccess
000613884 8564_ $$uhttps://bib-pubdb1.desy.de/record/613884/files/gkae374.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000613884 909CO $$ooai:bib-pubdb1.desy.de:613884$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000613884 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1023782$$aDeutsches Elektronen-Synchrotron$$b1$$kDESY
000613884 9101_ $$0I:(DE-H253)_CSSB-20140311$$6P:(DE-H253)PIP1023782$$aCentre for Structural Systems Biology$$b1$$kCSSB
000613884 9131_ $$0G:(DE-HGF)POF4-633$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vLife Sciences – Building Blocks of Life: Structure and Function$$x0
000613884 9141_ $$y2024
000613884 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-10-25
000613884 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-10-25
000613884 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000613884 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-10-25
000613884 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2023-10-25
000613884 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000613884 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2023-10-25
000613884 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-04-03T10:37:02Z
000613884 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-04-03T10:37:02Z
000613884 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2024-04-03T10:37:02Z
000613884 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNUCLEIC ACIDS RES : 2022$$d2024-12-20
000613884 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bNUCLEIC ACIDS RES : 2022$$d2024-12-20
000613884 9201_ $$0I:(DE-H253)FS-CS-20210408$$kFS-CS$$lStrukturelle Mikrobiologie CSSB$$x0
000613884 9201_ $$0I:(DE-H253)CSSB-LIV-KG-20220525$$kCSSB-LIV-KG$$lCSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald$$x1
000613884 980__ $$ajournal
000613884 980__ $$aVDB
000613884 980__ $$aUNRESTRICTED
000613884 980__ $$aI:(DE-H253)FS-CS-20210408
000613884 980__ $$aI:(DE-H253)CSSB-LIV-KG-20220525
000613884 9801_ $$aFullTexts
000613884 999C5 $$1Whitley$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0140-6736(00)04638-9$$p1513 -$$tLancet$$v357$$y2021
000613884 999C5 $$1Whitley$$2Crossref$$9-- missing cx lookup --$$a10.12688/f1000research.16157.1$$p1726 -$$tF1000Research$$v7$$y2018
000613884 999C5 $$1Bacon$$2Crossref$$9-- missing cx lookup --$$a10.1128/CMR.16.1.114-128.2003$$p114 -$$tClin. Microbiol. Rev.$$v16$$y2003
000613884 999C5 $$1Gilbert$$2Crossref$$9-- missing cx lookup --$$a10.1016/S1368-7646(02)00021-3$$p88 -$$tDrug Resist. Updat.$$v5$$y2002
000613884 999C5 $$1Howley$$2Crossref$$oHowley DNA Viruses 2022$$tDNA Viruses$$y2022
000613884 999C5 $$1James$$2Crossref$$9-- missing cx lookup --$$a10.2471/BLT.19.237149$$p315 -$$tBull. World Health Organ.$$v98$$y2020
000613884 999C5 $$1Rechenchoski$$2Crossref$$9-- missing cx lookup --$$a10.1007/s12223-016-0482-7$$p151 -$$tFolia Microbiol. (Praha)$$v62$$y2017
000613884 999C5 $$1Bradshaw$$2Crossref$$9-- missing cx lookup --$$a10.1007/s13311-016-0433-7$$p493 -$$tNeurotherapeutics$$v13$$y2016
000613884 999C5 $$1Elion$$2Crossref$$9-- missing cx lookup --$$a10.1126/science.2649979$$p41 -$$tScience$$v244$$y1989
000613884 999C5 $$1Grinde$$2Crossref$$9-- missing cx lookup --$$a10.3402/jom.v5i0.22766$$p22766 -$$tJ. Oral Microbiol.$$v5$$y2013
000613884 999C5 $$1Jha$$2Crossref$$9-- missing cx lookup --$$a10.3390/pathogens5010018$$p18 -$$tPathogens$$v5$$y2016
000613884 999C5 $$1Bjornevik$$2Crossref$$9-- missing cx lookup --$$a10.1126/science.abj8222$$p296 -$$tScience$$v375$$y2022
000613884 999C5 $$1Muylaert$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.R111.233981$$p15619 -$$tJ. Biol. Chem.$$v286$$y2011
000613884 999C5 $$1Elias$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.83.17.6322$$p6322 -$$tProc. Natl. Acad. Sci. U.S.A.$$v83$$y1986
000613884 999C5 $$1Olivo$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.85.15.5414$$p5414 -$$tProc. Natl. Acad. Sci. U.S.A.$$v85$$y1988
000613884 999C5 $$1Hernandez$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0021-9258(19)38580-1$$p11227 -$$tJ. Biol. Chem.$$v265$$y1990
000613884 999C5 $$1Gottlieb$$2Crossref$$9-- missing cx lookup --$$a10.1128/jvi.64.12.5976-5987.1990$$p5976 -$$tJ. Virol.$$v64$$y1990
000613884 999C5 $$1Lehman$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.274.40.28059$$p28059 -$$tJ. Biol. Chem.$$v274$$y1999
000613884 999C5 $$1Falkenberg$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.97.8.3896$$p3896 -$$tProc. Natl. Acad. Sci. U.S.A.$$v97$$y2000
000613884 999C5 $$1Stengel$$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.01688-10$$p957 -$$tJ. Virol.$$v85$$y2011
000613884 999C5 $$1Liu$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M602414200$$p18193 -$$tJ. Biol. Chem.$$v281$$y2006
000613884 999C5 $$1Hayes$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41467-021-23312-8$$p3040 -$$tNat. Commun.$$v12$$y2021
000613884 999C5 $$1Steitz$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.274.25.17395$$p17395 -$$tJ. Biol. Chem.$$v274$$y1999
000613884 999C5 $$1Zarrouk$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.virusres.2017.01.019$$p177 -$$tVirus Res.$$v234$$y2017
000613884 999C5 $$1Zuccola$$2Crossref$$9-- missing cx lookup --$$a10.1016/S1097-2765(00)80422-0$$p267 -$$tMol. Cell$$v5$$y2000
000613884 999C5 $$1Cohan$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.virusres.2021.198394$$p198394 -$$tVirus Res.$$v298$$y2021
000613884 999C5 $$1O’Donnell$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0021-9258(18)61340-7$$p4252 -$$tJ. Biol. Chem.$$v262$$y1987
000613884 999C5 $$1Zheng$$2Crossref$$9-- missing cx lookup --$$a10.1038/nmeth.4193$$p331 -$$tNat. Methods$$v14$$y2017
000613884 999C5 $$1Rohou$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.jsb.2015.08.008$$p216 -$$tJ. Struct. Biol.$$v192$$y2015
000613884 999C5 $$1Tegunov$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41592-019-0580-y$$p1146 -$$tNat. Methods$$v16$$y2019
000613884 999C5 $$1Zivanov$$2Crossref$$9-- missing cx lookup --$$a10.1107/S2052252520000081$$p253 -$$tIUCrJ$$v7$$y2020
000613884 999C5 $$1Punjani$$2Crossref$$9-- missing cx lookup --$$a10.1038/nmeth.4169$$p290 -$$tNat. Methods$$v14$$y2017
000613884 999C5 $$1Pettersen$$2Crossref$$9-- missing cx lookup --$$a10.1002/pro.3943$$p70 -$$tProtein Sci.$$v30$$y2021
000613884 999C5 $$1Kaur$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41467-021-21509-5$$p1240 -$$tNat. Commun.$$v12$$y2021
000613884 999C5 $$1Sanchez-Garcia$$2Crossref$$9-- missing cx lookup --$$a10.1038/s42003-021-02399-1$$p874 -$$tCommun. Biol.$$v4$$y2021
000613884 999C5 $$1Emsley$$2Crossref$$9-- missing cx lookup --$$a10.1107/S0907444904019158$$p2126 -$$tActa Crystallogr. D Biol. Crystallogr.$$v60$$y2004
000613884 999C5 $$1Croll$$2Crossref$$9-- missing cx lookup --$$a10.1107/S2059798318002425$$p519 -$$tActa Crystallogr. Sect. Struct. Biol.$$v74$$y2018
000613884 999C5 $$1Yamashita$$2Crossref$$9-- missing cx lookup --$$a10.1107/S2059798321009475$$p1282 -$$tActa Crystallogr. Sect. Struct. Biol.$$v77$$y2021
000613884 999C5 $$1Wang$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0092-8674(00)80296-2$$p1087 -$$tCell$$v89$$y1997
000613884 999C5 $$1Hogg$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M605675200$$p1432 -$$tJ. Biol. Chem.$$v282$$y2007
000613884 999C5 $$1Franklin$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0092-8674(01)00367-1$$p657 -$$tCell$$v105$$y2001
000613884 999C5 $$1Swan$$2Crossref$$9-- missing cx lookup --$$a10.1038/nsmb.1663$$p979 -$$tNat. Struct. Mol. Biol.$$v16$$y2009
000613884 999C5 $$1Guajardo$$2Crossref$$9-- missing cx lookup --$$a10.1006/jmbi.1996.0707$$p8 -$$tJ. Mol. Biol.$$v265$$y1997
000613884 999C5 $$1Berman$$2Crossref$$9-- missing cx lookup --$$a10.1038/sj.emboj.7601780$$p3494 -$$tEMBO J.$$v26$$y2007
000613884 999C5 $$1Brown$$2Crossref$$9-- missing cx lookup --$$a10.1021/bi101915z$$p1135 -$$tBiochemistry$$v50$$y2011
000613884 999C5 $$1Piret$$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.03360-14$$p4636 -$$tJ. Virol.$$v89$$y2015
000613884 999C5 $$1Vashishtha$$2Crossref$$9-- missing cx lookup --$$a10.1021/bi500840v$$p240 -$$tBiochemistry$$v54$$y2015
000613884 999C5 $$1Song$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M309848200$$p18535 -$$tJ. Biol. Chem.$$v279$$y2004
000613884 999C5 $$1Kühn$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.271.46.29245$$p29245 -$$tJ. Biol. Chem.$$v271$$y1996
000613884 999C5 $$1Hwang$$2Crossref$$9-- missing cx lookup --$$a10.1128/jvi.71.10.7791-7798.1997$$p7791 -$$tJ. Virol.$$v71$$y1997
000613884 999C5 $$1Beese$$2Crossref$$9-- missing cx lookup --$$a10.1002/j.1460-2075.1991.tb07917.x$$p25 -$$tEMBO J.$$v10$$y1991
000613884 999C5 $$1Steitz$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.90.14.6498$$p6498 -$$tProc. Natl. Acad. Sci. U.S.A.$$v90$$y1993
000613884 999C5 $$1Shamoo$$2Crossref$$9-- missing cx lookup --$$a10.1016/S0092-8674(00)81647-5$$p155 -$$tCell$$v99$$y1999
000613884 999C5 $$1Kunkel$$2Crossref$$9-- missing cx lookup --$$a10.1146/annurev.biochem.69.1.497$$p497 -$$tAnnu. Rev. Biochem.$$v69$$y2000
000613884 999C5 $$1Goodman$$2Crossref$$9-- missing cx lookup --$$a10.1093/genetics/148.4.1475$$p1475 -$$tGenetics$$v148$$y1998
000613884 999C5 $$1Punjani$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41592-023-01853-8$$p860 -$$tNat. Methods$$v20$$y2023
000613884 999C5 $$1Randell$$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.79.18.12025-12034.2005$$p12025 -$$tJ. Virol.$$v79$$y2005
000613884 999C5 $$1Kong$$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(92)90445-I$$p425 -$$tCell$$v69$$y1992
000613884 999C5 $$1Krishna$$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(94)90014-0$$p1233 -$$tCell$$v79$$y1994
000613884 999C5 $$1Komazin-Meredith$$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M708691200$$p6154 -$$tJ. Biol. Chem.$$v283$$y2008