000613872 001__ 613872
000613872 005__ 20250723172326.0
000613872 0247_ $$2doi$$a10.1101/2024.02.14.580233
000613872 0247_ $$2datacite_doi$$a10.3204/PUBDB-2024-05651
000613872 0247_ $$2openalex$$aopenalex:W4391841916
000613872 037__ $$aPUBDB-2024-05651
000613872 041__ $$aEnglish
000613872 1001_ $$aGolan, Nimrod$$b0
000613872 245__ $$aResilience and Charge-Dependent Fibrillation of Functional Amyloids: Interactions of Pseudomonas Biofilm-Associated FapB and FapC
000613872 260__ $$c2024
000613872 3367_ $$0PUB:(DE-HGF)25$$2PUB:(DE-HGF)$$aPreprint$$bpreprint$$mpreprint$$s1728309909_93053
000613872 3367_ $$2ORCID$$aWORKING_PAPER
000613872 3367_ $$028$$2EndNote$$aElectronic Article
000613872 3367_ $$2DRIVER$$apreprint
000613872 3367_ $$2BibTeX$$aARTICLE
000613872 3367_ $$2DataCite$$aOutput Types/Working Paper
000613872 520__ $$aFapC and FapB are biofilm-associated amyloids involved in the virulence of Pseudomonas and other bacteria. We herein demonstrate their exceptional thermal and chemical resilience, suggesting that biofilm structures might withstand standard sterilization, thereby contributing to the persistence of P. aeruginosa infections. Our findings also underscore the impact of environmental factors on Fap proteins, suggesting that orthologs in different Pseudomonas strains adapt to specific environments and roles. Challenging previous assumptions about a simple nucleation role for FapB in promoting FapC aggregation, the study shows a significant influence of FapC on FapB aggregation. The interaction between FapB and FapC is intricate: FapB stabilizes FapC fibrils, while FapC slows down FapB fibrillation but can still serve as a cross-seeding template. This complex interplay is key to understanding their roles in bacterial biofilms. Furthermore, the study highlights distinct differences between Fap and E. coli’s curli CsgA amyloid, where CsgB assumes a simple unidirectional role in nucleating CsgA fibrillation, emphasizing the importance of a comprehensive understanding of various amyloid systems. This knowledge is vital for developing effective intervention strategies against bacterial infections and leveraging the unique properties of these amyloids in technological applications such as novel bio-nanomaterials or protective coatings.
000613872 536__ $$0G:(DE-HGF)POF4-633$$a633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633)$$cPOF4-633$$fPOF IV$$x0
000613872 588__ $$aDataset connected to CrossRef
000613872 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0
000613872 7001_ $$aParizat, Amit$$b1
000613872 7001_ $$aTabachnikov, Orly$$b2
000613872 7001_ $$aBarnea, Eilon$$b3
000613872 7001_ $$aOlsen, William P.$$b4
000613872 7001_ $$aOtzen, Daniel E.$$b5
000613872 7001_ $$0P:(DE-H253)PIP1026961$$aLandau, Meytal$$b6$$eCorresponding author$$udesy
000613872 773__ $$a10.1101/2024.02.14.580233
000613872 8564_ $$uhttps://bib-pubdb1.desy.de/record/613872/files/Resilience_and_Charge-Dependent_Fibrillation_of_Fu.pdf$$yOpenAccess
000613872 8564_ $$uhttps://bib-pubdb1.desy.de/record/613872/files/Resilience_and_Charge-Dependent_Fibrillation_of_Fu.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000613872 909CO $$ooai:bib-pubdb1.desy.de:613872$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000613872 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000613872 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000613872 915__ $$0StatID:(DE-HGF)0580$$2StatID$$aPublished
000613872 9141_ $$y2024
000613872 9101_ $$0I:(DE-H253)_CSSB-20140311$$6P:(DE-H253)PIP1026961$$aCentre for Structural Systems Biology$$b6$$kCSSB
000613872 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1026961$$aEuropean Molecular Biology Laboratory$$b6$$kEMBL
000613872 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1026961$$aDeutsches Elektronen-Synchrotron$$b6$$kDESY
000613872 9131_ $$0G:(DE-HGF)POF4-633$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vLife Sciences – Building Blocks of Life: Structure and Function$$x0
000613872 9201_ $$0I:(DE-H253)CSSB-F-20230420$$kCSSB-F$$lFPF Functional Protein Fibrils$$x0
000613872 980__ $$apreprint
000613872 980__ $$aVDB
000613872 980__ $$aUNRESTRICTED
000613872 980__ $$aI:(DE-H253)CSSB-F-20230420
000613872 9801_ $$aFullTexts