000613834 001__ 613834 000613834 005__ 20250723172323.0 000613834 0247_ $$2doi$$a10.7554/eLife.87726 000613834 0247_ $$2datacite_doi$$a10.3204/PUBDB-2024-05640 000613834 0247_ $$2altmetric$$aaltmetric:158752043 000613834 0247_ $$2pmid$$apmid:38270586 000613834 0247_ $$2openalex$$aopenalex:W4379875417 000613834 037__ $$aPUBDB-2024-05640 000613834 041__ $$aEnglish 000613834 082__ $$a600 000613834 1001_ $$00000-0003-2461-5256$$aAndradi-Brown, Clare$$b0 000613834 245__ $$aA novel computational pipeline for var gene expression augments the discovery of changes in the Plasmodium falciparum transcriptome during transition from in vivo to short-term in vitro culture 000613834 260__ $$c2024 000613834 3367_ $$0PUB:(DE-HGF)25$$2PUB:(DE-HGF)$$aPreprint$$bpreprint$$mpreprint$$s1730104256_1019402 000613834 3367_ $$2ORCID$$aWORKING_PAPER 000613834 3367_ $$028$$2EndNote$$aElectronic Article 000613834 3367_ $$2DRIVER$$apreprint 000613834 3367_ $$2BibTeX$$aARTICLE 000613834 3367_ $$2DataCite$$aOutput Types/Working Paper 000613834 520__ $$aThe pathogenesis of severe Plasmodium falciparum malaria involves cytoadhesive microvascular sequestration of infected erythrocytes, mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1). PfEMP1 variants are encoded by the highly polymorphic family of var genes, the sequences of which are largely unknown in clinical samples. Previously, we published new approaches for var gene profiling and classification of predicted binding phenotypes in clinical P. falciparum isolates (Wichers et al., 2021), which represented a major technical advance. Building on this, we report here a novel method for var gene assembly and multidimensional quantification from RNA-sequencing that outperforms the earlier approach of Wichers et al., 2021, on both laboratory and clinical isolates across a combination of metrics. Importantly, the tool can interrogate the var transcriptome in context with the rest of the transcriptome and can be applied to enhance our understanding of the role of var genes in malaria pathogenesis. We applied this new method to investigate changes in var gene expression through early transition of parasite isolates to in vitro culture, using paired sets of ex vivo samples from our previous study, cultured for up to three generations. In parallel, changes in non-polymorphic core gene expression were investigated. Modest but unpredictable var gene switching and convergence towards var2csa were observed in culture, along with differential expression of 19% of the core transcriptome between paired ex vivo and generation 1 samples. Our results cast doubt on the validity of the common practice of using short-term cultured parasites to make inferences about in vivo phenotype and behaviour. 000613834 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0 000613834 536__ $$0G:(GEPRIS)323759012$$aDFG project G:(GEPRIS)323759012 - Analyse der Transkriptionsdynamik von Virulenzfaktoren in klinischen Isolaten des Malariaerregers Plasmodium falciparum (323759012)$$c323759012$$x1 000613834 536__ $$0G:(GEPRIS)433302244$$aDFG project G:(GEPRIS)433302244 - Epigenetische Regulation der Antigenvariation im Malariaerreger Plasmodium falciparum (433302244)$$c433302244$$x2 000613834 542__ $$2Crossref$$i2024-01-25$$uhttp://creativecommons.org/licenses/by/4.0/ 000613834 542__ $$2Crossref$$i2024-01-25$$uhttp://creativecommons.org/licenses/by/4.0/ 000613834 542__ $$2Crossref$$i2024-01-25$$uhttp://creativecommons.org/licenses/by/4.0/ 000613834 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de 000613834 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0 000613834 7001_ $$00000-0002-0599-1742$$aWichers-Misterek, Jan Stephan$$b1 000613834 7001_ $$0P:(DE-HGF)0$$avon Thien, Heidrun$$b2 000613834 7001_ $$0P:(DE-HGF)0$$aHöppner, Yannick D$$b3 000613834 7001_ $$0P:(DE-HGF)0$$aScholz, Judith AM$$b4 000613834 7001_ $$00000-0001-6484-1165$$aHansson, Helle$$b5 000613834 7001_ $$0P:(DE-HGF)0$$aFiltenborg Hocke, Emma$$b6 000613834 7001_ $$0P:(DE-H253)PIP1023784$$aGilberger, Tim$$b7 000613834 7001_ $$0P:(DE-HGF)0$$aDuffy, Michael F$$b8 000613834 7001_ $$00000-0002-3044-4249$$aLavstsen, Thomas$$b9 000613834 7001_ $$0P:(DE-HGF)0$$aBaum, Jake$$b10 000613834 7001_ $$00000-0002-1246-7404$$aOtto, Thomas D$$b11 000613834 7001_ $$0P:(DE-HGF)0$$aCunnington, Aubrey J.$$b12$$eCorresponding author 000613834 7001_ $$0P:(DE-HGF)0$$aBachmann, Anna$$b13$$eCorresponding author 000613834 77318 $$2Crossref$$3journal-article$$a10.7554/elife.87726$$beLife Sciences Publications, Ltd$$d2024-01-25$$teLife$$v12$$x2050-084X$$y2024 000613834 773__ $$0PERI:(DE-600)2687154-3$$a10.7554/eLife.87726$$gVol. 12, p. RP87726$$teLife$$v12$$x2050-084X$$y2024 000613834 8564_ $$uhttps://bib-pubdb1.desy.de/record/613834/files/87726-v2.pdf$$yOpenAccess 000613834 8564_ $$uhttps://bib-pubdb1.desy.de/record/613834/files/87726-v2.pdf?subformat=pdfa$$xpdfa$$yOpenAccess 000613834 909CO $$ooai:bib-pubdb1.desy.de:613834$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire 000613834 9101_ $$0I:(DE-H253)_CSSB-20140311$$6P:(DE-H253)PIP1023784$$aCentre for Structural Systems Biology$$b7$$kCSSB 000613834 9131_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0 000613834 9141_ $$y2024 000613834 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-08-22 000613834 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-08-22 000613834 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-08-22 000613834 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2023-08-22 000613834 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0 000613834 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess 000613834 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2023-08-22 000613834 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bELIFE : 2022$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-07-30T13:58:16Z 000613834 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-07-30T13:58:16Z 000613834 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2024-07-30T13:58:16Z 000613834 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-28 000613834 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bELIFE : 2022$$d2024-12-28 000613834 9201_ $$0I:(DE-H253)CSSB-BNITM-TG-20210520$$kCSSB-BNITM-TG$$lCSSB-BNITM-TG$$x0 000613834 980__ $$apreprint 000613834 980__ $$aVDB 000613834 980__ $$aI:(DE-H253)CSSB-BNITM-TG-20210520 000613834 980__ $$aUNRESTRICTED 000613834 9801_ $$aFullTexts 000613834 999C5 $$1Ahouidi$$2Crossref$$9-- missing cx lookup --$$a10.12688/wellcomeopenres.16168.2$$tWellcome Open Research$$v6$$y2021 000613834 999C5 $$1Almelli$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0114401$$tPLOS ONE$$v9$$y2014 000613834 999C5 $$1Anders$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btu638$$p166 -$$tBioinformatics$$v31$$y2015 000613834 999C5 $$1Andrade$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41591-020-1084-0$$p1929 -$$tNature Medicine$$v26$$y2020 000613834 999C5 $$1Andreadaki$$2Crossref$$9-- missing cx lookup --$$a10.1002/mbo3.1038$$tMicrobiologyOpen$$v9$$y2020 000613834 999C5 $$1Avril$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1120534109$$pE1782 -$$tPNAS$$v109$$y2012 000613834 999C5 $$1Bachmann$$2Crossref$$9-- missing cx lookup --$$a10.1111/j.1462-5822.2011.01629.x$$p1397 -$$tCellular Microbiology$$v13$$y2011 000613834 999C5 $$1Baruch$$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(95)90054-3$$p77 -$$tCell$$v82$$y1995 000613834 999C5 $$1Beeson$$2Crossref$$9-- missing cx lookup --$$a10.1093/femsre/fuw001$$p343 -$$tFEMS Microbiology Reviews$$v40$$y2016 000613834 999C5 $$1Bernabeu$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1524294113$$pE3270 -$$tPNAS$$v113$$y2016 000613834 999C5 $$1Boetzer$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btq683$$p578 -$$tBioinformatics$$v27$$y2011 000613834 999C5 $$1Bozdech$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pbio.0000005$$tPLOS Biology$$v1$$y2003 000613834 999C5 $$1Brown$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.pt.2020.08.008$$p914 -$$tTrends in Parasitology$$v36$$y2020 000613834 999C5 $$1Brown$$2Crossref$$tSoftware Heritage$$uBrown CA. 2023. Varassembly. Software Heritage. swh:1:rev:1f7aeeca5d97427dbd54cb6cfb54c9e6322a8fea. https://archive.softwareheritage.org/swh:1:dir:9cfd6ffe0a27f5a09e256cb56dbcffb60d1930e4;origin=https://github.com/ClareAndradiBrown/varAssembly;visit=swh:1:snp:9a6f70069bf9e9ed6a54b05372518a60a724cb7b;anchor=swh:1:rev:1f7aeeca5d97427dbd54cb6cfb54c9e6322a8fea.$$y2023 000613834 999C5 $$1Bruske$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0166135$$tPLOS ONE$$v11$$y2016 000613834 999C5 $$1Bushmanova$$2Crossref$$9-- missing cx lookup --$$a10.1093/gigascience/giz100$$tGigaScience$$v8$$y2019 000613834 999C5 $$1Carrington$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.isci.2021.102444$$tiScience$$v24$$y2021 000613834 999C5 $$1Claessens$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1120461109$$pE1772 -$$tPNAS$$v109$$y2012 000613834 999C5 $$1Claessens$$2Crossref$$9-- missing cx lookup --$$a10.1038/srep41303$$tScientific Reports$$v7$$y2017 000613834 999C5 $$1Danecek$$2Crossref$$9-- missing cx lookup --$$a10.1093/gigascience/giab008$$tGigaScience$$v10$$y2021 000613834 999C5 $$1Dara$$2Crossref$$9-- missing cx lookup --$$a10.1186/s13073-017-0422-4$$tGenome Medicine$$v9$$y2017 000613834 999C5 $$1Dimonte$$2Crossref$$9-- missing cx lookup --$$a10.1093/infdis/jiw225$$p884 -$$tThe Journal of Infectious Diseases$$v214$$y2016 000613834 999C5 $$1Dimonte$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12936-020-03257-x$$tMalaria Journal$$v19$$y2020 000613834 999C5 $$1Dong$$2Crossref$$9-- missing cx lookup --$$a10.1093/bib/bbz166$$p416 -$$tBriefings in Bioinformatics$$v22$$y2021 000613834 999C5 $$1Duffy$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12936-016-1296-4$$tMalaria Journal$$v15$$y2016 000613834 999C5 $$1Finn$$2Crossref$$9-- missing cx lookup --$$a10.1093/nar/gkr367$$pW29 -$$tNucleic Acids Research$$v39$$y2011 000613834 999C5 $$1Fu$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/bts565$$p3150 -$$tBioinformatics$$v28$$y2012 000613834 999C5 $$1Ge$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btz931$$p2628 -$$tBioinformatics$$v36$$y2020 000613834 999C5 $$1Guillochon$$2Crossref$$9-- missing cx lookup --$$a10.1093/infdis/jiac086$$p2187 -$$tThe Journal of Infectious Diseases$$v225$$y2022 000613834 999C5 $$1Henschel$$2Crossref$$9-- missing cx lookup --$$a10.1145/2335755.2335842$$p1 -$$uHenschel R, Lieber M, Wu LS, Nista PM, Haas BJ, LeDuc RD. 2012. Trinity RNA-Seq assembler performance optimization. XSEDE12.$$y2012 000613834 999C5 $$1Hoo$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.ebiom.2019.09.001$$p442 -$$tEBioMedicine$$v48$$y2019 000613834 999C5 $$1Howick$$2Crossref$$9-- missing cx lookup --$$a10.1126/science.aaw2619$$tScience$$v365$$y2019 000613834 999C5 $$1Huang$$2Crossref$$9-- missing cx lookup --$$a10.1101/gr.9.9.868$$p868 -$$tGenome Research$$v9$$y1999 000613834 999C5 $$1Jensen$$2Crossref$$9-- missing cx lookup --$$a10.1084/jem.20040274$$p1179 -$$tThe Journal of Experimental Medicine$$v199$$y2004 000613834 999C5 $$1Jespersen$$2Crossref$$9-- missing cx lookup --$$a10.15252/emmm.201606188$$p839 -$$tEMBO Molecular Medicine$$v8$$y2016 000613834 999C5 $$1Joste$$2Crossref$$9-- missing cx lookup --$$a10.1128/mBio.02103-20$$tmBio$$v11$$y2020 000613834 999C5 $$1Kaneko$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1004905$$tPLOS Pathogens$$v11$$y2015 000613834 999C5 $$1Kessler$$2Crossref$$9-- missing cx lookup --$$a10.1016/j.chom.2017.09.009$$p601 -$$tCell Host & Microbe$$v22$$y2017 000613834 999C5 $$1Kirchgatter$$2Crossref$$9-- missing cx lookup --$$a10.1007/BF03401999$$p16 -$$tMolecular Medicine$$v8$$y2002 000613834 999C5 $$1Kraemer$$2Crossref$$9-- missing cx lookup --$$a10.1046/j.1365-2958.2003.03814.x$$p1527 -$$tMolecular Microbiology$$v50$$y2003 000613834 999C5 $$1Kyes$$2Crossref$$9-- missing cx lookup --$$a10.1128/EC.00173-07$$p1511 -$$tEukaryotic Cell$$v6$$y2007 000613834 999C5 $$1Lavstsen$$2Crossref$$9-- missing cx lookup --$$a10.1186/1475-2875-2-27$$tMalaria Journal$$v2$$y2003 000613834 999C5 $$1Lavstsen$$2Crossref$$9-- missing cx lookup --$$a10.1186/1475-2875-4-21$$tMalaria Journal$$v4$$y2005 000613834 999C5 $$1Lavstsen$$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1120455109$$pE1791 -$$tPNAS$$v109$$y2012 000613834 999C5 $$1Lee$$2Crossref$$9-- missing cx lookup --$$a10.1126/scitranslmed.aar3619$$tScience Translational Medicine$$v10$$y2018 000613834 999C5 $$1Leech$$2Crossref$$9-- missing cx lookup --$$a10.1084/jem.159.6.1567$$p1567 -$$tThe Journal of Experimental Medicine$$v159$$y1984 000613834 999C5 $$1Lewis$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/bty863$$p1766 -$$tBioinformatics$$v35$$y2019 000613834 999C5 $$1Li$$2Crossref$$tarXiv$$uLi H. 2013. Aligning Sequence Reads, Clone Sequences and Assembly Contigs with BWA-MEM. arXiv. https://arxiv.org/abs/1303.3997.$$y2013 000613834 999C5 $$1Liao$$2Crossref$$9-- missing cx lookup --$$a10.1093/nar/gkt214$$tNucleic Acids Research$$v41$$y2013 000613834 999C5 $$1Liao$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btt656$$p923 -$$tBioinformatics$$v30$$y2014 000613834 999C5 $$1Lischer$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12859-017-1911-6$$tBMC Bioinformatics$$v18$$y2017 000613834 999C5 $$1Love$$2Crossref$$9-- missing cx lookup --$$a10.1186/s13059-014-0550-8$$tGenome Biology$$v15$$y2014 000613834 999C5 $$1Mackenzie$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12859-022-04573-6$$tBMC Bioinformatics$$v23$$y2022 000613834 999C5 $$1Mackinnon$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1000644$$tPLOS Pathogens$$v5$$y2009 000613834 999C5 $$1Mkumbaye$$2Crossref$$9-- missing cx lookup --$$a10.1128/IAI.00841-16$$tInfection and Immunity$$v85$$y2017 000613834 999C5 $$1Mok$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0001982$$tPLOS ONE$$v3$$y2008 000613834 999C5 $$1Oakley$$2Crossref$$9-- missing cx lookup --$$a10.1128/IAI.01236-06$$p2012 -$$tInfection and Immunity$$v75$$y2007 000613834 999C5 $$1Otto$$2Crossref$$9-- missing cx lookup --$$a10.12688/wellcomeopenres.15590.1$$tWellcome Open Research$$v4$$y2019 000613834 999C5 $$1Patro$$2Crossref$$9-- missing cx lookup --$$a10.1038/nmeth.4197$$p417 -$$tNature Methods$$v14$$y2017 000613834 999C5 $$1Peters$$2Crossref$$9-- missing cx lookup --$$a10.1086/511436$$p748 -$$tThe Journal of Infectious Diseases$$v195$$y2007 000613834 999C5 $$1Pickford$$2Crossref$$9-- missing cx lookup --$$a10.1128/mBio.01636-21$$tmBio$$v12$$y2021 000613834 999C5 $$1Quintana$$2Crossref$$9-- missing cx lookup --$$a10.1038/s41598-019-42585-0$$tScientific Reports$$v9$$y2019 000613834 999C5 $$1Rask$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pcbi.1000933$$tPLOS Computational Biology$$v6$$y2010 000613834 999C5 $$1Robert$$2Crossref$$9-- missing cx lookup --$$a10.1016/s0035-9203(96)90446-0$$p704 -$$tTransactions of the Royal Society of Tropical Medicine and Hygiene$$v90$$y1996 000613834 999C5 $$1Rorick$$2Crossref$$9-- missing cx lookup --$$a10.1186/1471-2180-13-244$$tBMC Microbiology$$v13$$y2013 000613834 999C5 $$1Sahu$$2Crossref$$9-- missing cx lookup --$$a10.1172/jci.insight.145823$$tJCI Insight$$v6$$y2021 000613834 999C5 $$1Salanti$$2Crossref$$9-- missing cx lookup --$$a10.1084/jem.20041579$$p1197 -$$tThe Journal of Experimental Medicine$$v200$$y2004 000613834 999C5 $$1Scherf$$2Crossref$$9-- missing cx lookup --$$a10.1093/emboj/17.18.5418$$p5418 -$$tThe EMBO Journal$$v17$$y1998 000613834 999C5 $$1Schulz$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/bts094$$p1086 -$$tBioinformatics$$v28$$y2012 000613834 999C5 $$1Shabani$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12916-017-0945-y$$tBMC Medicine$$v15$$y2017 000613834 999C5 $$1Smith$$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(95)90056-x$$p101 -$$tCell$$v82$$y1995 000613834 999C5 $$1Stevenson$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1005022$$tPLOS Pathogens$$v11$$y2015 000613834 999C5 $$1Storm$$2Crossref$$9-- missing cx lookup --$$a10.15252/emmm.201809164$$tEMBO Molecular Medicine$$v11$$y2019 000613834 999C5 $$1Stucke$$2Crossref$$9-- missing cx lookup --$$a10.1128/mSystems.00226-21$$tmSystems$$v6$$y2021 000613834 999C5 $$1Su$$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(95)90055-1$$p89 -$$tCell$$v82$$y1995 000613834 999C5 $$1Tarr$$2Crossref$$9-- missing cx lookup --$$a10.1186/s12864-018-5257-x$$tBMC Genomics$$v19$$y2018 000613834 999C5 $$1Taylor$$2Crossref$$9-- missing cx lookup --$$a10.1128/IAI.70.10.5779-5789.2002$$p5779 -$$tInfection and Immunity$$v70$$y2002 000613834 999C5 $$1Tebben$$2Crossref$$9-- missing cx lookup --$$a10.1128/msystems.00258-22$$tmSystems$$v7$$y2022 000613834 999C5 $$1Tonkin-Hill$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pbio.2004328$$tPLOS Biology$$v16$$y2018 000613834 999C5 $$1Tuikue Ndam$$2Crossref$$9-- missing cx lookup --$$a10.1093/infdis/jix230$$p1918 -$$tThe Journal of Infectious Diseases$$v215$$y2017 000613834 999C5 $$1Turner$$2Crossref$$9-- missing cx lookup --$$a10.1038/nature12216$$p502 -$$tNature$$v498$$y2013 000613834 999C5 $$1Ukaegbu$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1003854$$tPLOS Pathogens$$v10$$y2014 000613834 999C5 $$1Ukaegbu$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pgen.1005234$$tPLOS Genetics$$v11$$y2015 000613834 999C5 $$1Vignali$$2Crossref$$9-- missing cx lookup --$$a10.1172/JCI43457$$p1119 -$$tThe Journal of Clinical Investigation$$v121$$y2011 000613834 999C5 $$1Wagner$$2Crossref$$9-- missing cx lookup --$$a10.3389/fmicb.2018.01037$$tFrontiers in Microbiology$$v9$$y2018 000613834 999C5 $$1Wahlgren$$2Crossref$$9-- missing cx lookup --$$a10.1038/nrmicro.2017.47$$p479 -$$tNature Reviews. Microbiology$$v15$$y2017 000613834 999C5 $$1Wang$$2Crossref$$tarXiv$$uWang W, Chen EZ, Li H. 2021. Truncated rank-based tests for two-part models with excessive zeros and applications to microbiome data. arXiv. https://arxiv.org/abs/2110.05368.$$y2021 000613834 999C5 $$1Warimwe$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0070467$$tPLOS ONE$$v8$$y2013 000613834 999C5 $$1WHO$$2Crossref$$tWorld malaria report 2022$$uWHO. 2022. Geneva: World Health Organization. World malaria report 2022.$$y2022 000613834 999C5 $$1Wichers$$2Crossref$$9-- missing cx lookup --$$a10.1128/mBio.01500-19$$tmBio$$v10$$y2019 000613834 999C5 $$1Wichers$$2Crossref$$9-- missing cx lookup --$$a10.7554/eLife.69040$$teLife$$v10$$y2021 000613834 999C5 $$1Xie$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btu077$$p1660 -$$tBioinformatics$$v30$$y2014 000613834 999C5 $$1Yamagishi$$2Crossref$$9-- missing cx lookup --$$a10.1101/gr.158980.113$$p1433 -$$tGenome Research$$v24$$y2014 000613834 999C5 $$1Yang$$2Crossref$$9-- missing cx lookup --$$a10.1186/1471-2164-13-475$$tBMC Genomics$$v13$$y2012 000613834 999C5 $$1Yuda$$2Crossref$$9-- missing cx lookup --$$a10.1111/j.1365-2958.2009.07005.x$$p854 -$$tMolecular Microbiology$$v75$$y2010 000613834 999C5 $$1Zerbino$$2Crossref$$9-- missing cx lookup --$$a10.1101/gr.074492.107$$p821 -$$tGenome Research$$v18$$y2008 000613834 999C5 $$1Zhang$$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0020591$$tPLOS ONE$$v6$$y2011 000613834 999C5 $$1Zhang$$2Crossref$$9-- missing cx lookup --$$a10.7554/eLife.83840$$teLife$$v11$$y2022 000613834 999C5 $$1Zimin$$2Crossref$$9-- missing cx lookup --$$a10.1093/bioinformatics/btt476$$p2669 -$$tBioinformatics$$v29$$y2013