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@ARTICLE{Siggel:613831,
author = {Siggel, Marc and Jensen, Rasmus K. and Maurer, Valentin J.
and Mahamid, Julia and Kosinski, Jan},
title = {{C}olab{S}eg: {A}n interactive tool for editing,
processing, and visualizing membrane segmentations from
cryo-{ET} data},
journal = {Journal of structural biology},
volume = {216},
number = {2},
issn = {1047-8477},
address = {San Diego, Calif.},
publisher = {Elsevier},
reportid = {PUBDB-2024-05637},
pages = {108067},
year = {2024},
abstract = {Cellular cryo-electron tomography (cryo-ET) has emerged as
a key method to unravel the spatial and structural
complexity of cells in their near-native state at
unprecedented molecular resolution. To enable quantitative
analysis of the complex shapes and morphologies of lipid
membranes, the noisy three-dimensional (3D) volumes must be
segmented. Despite recent advances, this task often requires
considerable user intervention to curate the resulting
segmentations. Here, we present ColabSeg, a Python-based
tool for processing, visualizing, editing, and fitting
membrane segmentations from cryo-ET data for downstream
analysis. ColabSeg makes many well-established algorithms
for point-cloud processing easily available to the broad
community of structural biologists for applications in
cryo-ET through its graphical user interface (GUI). We
demonstrate the usefulness of the tool with a range of use
cases and biological examples. Finally, for a large
Mycoplasma pneumoniae dataset of 50 tomograms, we show how
ColabSeg enables high-throughput membrane segmentation,
which can be used as valuable training data for fully
automated convolutional neural network (CNN)-based
segmentation.},
cin = {CSSB-EMBL-JK},
ddc = {540},
cid = {I:(DE-H253)CSSB-EMBL-JK-20210701},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {38367824},
UT = {WOS:001205861300001},
doi = {10.1016/j.jsb.2024.108067},
url = {https://bib-pubdb1.desy.de/record/613831},
}