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@ARTICLE{Franzkoch:613729,
      author       = {Franzkoch, Rico and Anand, Aby and Breitsprecher, Leonhard
                      and Psathaki, Olympia E. and Barisch, Caroline},
      title        = {{R}esolving exit strategies of mycobacteria in
                      {D}ictyostelium discoideum by combining high‐pressure
                      freezing with 3{D} ‐correlative light and electron
                      microscopy},
      journal      = {Molecular microbiology},
      volume       = {121},
      number       = {3},
      issn         = {0950-382X},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {PUBDB-2024-05617},
      pages        = {593-604},
      year         = {2024},
      abstract     = {The infection course of Mycobacterium tuberculosis is
                      highly dynamic and comprises sequential stages that require
                      damaging and crossing of several membranes to enable the
                      translocation of the bacteria into the cytosol or their
                      escape from the host. Many important breakthroughs such as
                      the restriction of mycobacteria by the autophagy pathway and
                      the recruitment of sophisticated host repair machineries to
                      the Mycobacterium-containing vacuole have been gained in the
                      Dictyostelium discoideum/M. marinum system. Despite the
                      availability of well-established light and advanced electron
                      microscopy techniques in this system, a correlative approach
                      integrating both methods with near-native ultrastructural
                      preservation is currently lacking. This is most likely due
                      to the low ability of D. discoideum to adhere to surfaces,
                      which results in cell loss even after fixation. To address
                      this problem, we improved the adhesion of cells and
                      developed a straightforward and convenient workflow for
                      3D-correlative light and electron microscopy. This approach
                      includes high-pressure freezing, which is an excellent
                      technique for preserving membranes. Thus, our method allows
                      to monitor the ultrastructural aspects of vacuole escape
                      which is of central importance for the survival and
                      dissemination of bacterial pathogens.},
      cin          = {CSSB-FZB-CB},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-FZB-CB-20230819},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project
                      G:(GEPRIS)531703706 - Entschlüsselung der molekularen
                      Mechanismen des Austritts von Orientia tsutsugamushi aus der
                      Wirtszelle (531703706) / SFB 1557 P01 - Umbau und Nutzung
                      der Lipid-Trafficking-Maschinerie der Wirtszelle durch
                      pathogene Mykobakterien (P01) (516902060) / SFB 944 P25 -
                      Funktionale Bedeutung des Lipidtransports für die
                      mykobakterielle Infektion in Dictyostelium (P25)
                      (442222172)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)531703706 /
                      G:(GEPRIS)516902060 / G:(GEPRIS)442222172},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38063129},
      UT           = {WOS:001119243700001},
      doi          = {10.1111/mmi.15205},
      url          = {https://bib-pubdb1.desy.de/record/613729},
}