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@ARTICLE{Koua:611574,
      author       = {Koua, Faisal Hammad Mekky and Han, Huijong and Bean,
                      Richard},
      title        = {{S}tructural {D}ynamics of {M}etalloproteins and {R}edox
                      {E}nzymology with {M}ix-and-{I}nject {T}ime-{R}esolved
                      {S}erial {F}emtosecond {C}rystallography},
      journal      = {ACS catalysis},
      volume       = {14},
      number       = {14},
      issn         = {2155-5435},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {PUBDB-2024-04984},
      pages        = {10853-10870},
      year         = {2024},
      abstract     = {Time-resolved serial femtosecond crystallography (tr-SFX)
                      enables the study of biomolecules in action at room
                      temperature, thus facilitating the construction of in
                      crystallo mini-3D biomolecular series (a stop-motion series)
                      of biochemical reactions with unprecedented spatiotemporal
                      details. One of the challenges encountered in expanding this
                      method is the insufficient tools available for triggering
                      the biomacromolecular reaction. Here, we highlight recent
                      advances and challenges in the mix-and-inject
                      (diffusion-based) tr-SFX (MISC) as a promising triggering
                      method for studying the structural dynamics of
                      metalloproteins, redox enzymes, and their reaction kinetics.
                      We further discuss the results obtained using MISC tr-SFX
                      and propose complex MISC (cMISC) as a tool to study complex
                      reaction kinetics such as theenzyme-catalyzed bisubstrate
                      (sequential and ping-pong) reactions.},
      cin          = {$XFEL_E1_SPB/SFX$},
      ddc          = {540},
      cid          = {$I:(DE-H253)XFEL_E1_SPB_SFX-20210408$},
      pnm          = {6G13 - Accelerator of European XFEL (POF4-6G13)},
      pid          = {G:(DE-HGF)POF4-6G13},
      experiment   = {EXP:(DE-H253)XFEL-Exp-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:001263987000001},
      doi          = {10.1021/acscatal.4c02526},
      url          = {https://bib-pubdb1.desy.de/record/611574},
}