guest ::
| Home > Publications database > 3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi > print |
| Home > Publications database > 3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi > print |
| 001 | 611461 | ||
| 005 | 20250723171908.0 | ||
| 024 | 7 | _ | |a 10.1002/bit.28788 |2 doi |
| 024 | 7 | _ | |a 0006-3592 |2 ISSN |
| 024 | 7 | _ | |a 0572-6565 |2 ISSN |
| 024 | 7 | _ | |a 1097-0290 |2 ISSN |
| 024 | 7 | _ | |a 10.3204/PUBDB-2024-04926 |2 datacite_doi |
| 024 | 7 | _ | |a 38943490 |2 pmid |
| 024 | 7 | _ | |a WOS:001258645700001 |2 WOS |
| 024 | 7 | _ | |a openalex:W4400151777 |2 openalex |
| 037 | _ | _ | |a PUBDB-2024-04926 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Dinius, Anna |0 P:(DE-H253)PIP1100470 |b 0 |
| 245 | _ | _ | |a 3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi |
| 260 | _ | _ | |a New York, NY |c 2024 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1728652829_768677 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a ISSN 1097-0290 not unique: **2 hits**. |
| 520 | _ | _ | |a Controlling the morphology of filamentous fungi is crucial to improve the performance of fungal bioprocesses. Microparticle-enhanced cultivation (MPEC) increases productivity, most likely by changing the fungal morphology. However, due to a lack of appropriate methods, the exact impact of the added microparticles on the structural development of fungal pellets is mostly unexplored. In this study synchrotron radiation-based microcomputed tomography and three-dimensional (3D) image analysis were applied to unveil the detailed 3D incorporation of glass microparticles in nondestructed pellets of Aspergillus niger from MPEC. The developed method enabled the 3D analysis based on 375 pellets from various MPEC experiments. The total and locally resolved volume fractions of glass microparticles and hyphae were quantified for the first time. At increasing microparticle concentrations in the culture medium, pellets with lower hyphal fraction were obtained. However, the total volume of incorporated glass microparticles within the pellets did not necessarily increase. Furthermore, larger microparticles were less effective than smaller ones in reducing pellet density. However, the total volume of incorporated glass was larger for large microparticles. In addition, analysis of MPEC pellets from different times of cultivation indicated that spore agglomeration is decisive for the development of MPEC pellets. The developed 3D morphometric analysis method and the presented results will promote the general understanding and further development of MPEC for industrial application. |
| 536 | _ | _ | |a 6G3 - PETRA III (DESY) (POF4-6G3) |0 G:(DE-HGF)POF4-6G3 |c POF4-6G3 |f POF IV |x 0 |
| 536 | _ | _ | |a FS-Proposal: I-20211150 (I-20211150) |0 G:(DE-H253)I-20211150 |c I-20211150 |x 1 |
| 536 | _ | _ | |a FS-Proposal: I-20200214 (I-20200214) |0 G:(DE-H253)I-20200214 |c I-20200214 |x 2 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)427889137 - Kontrolle von Monospezies und Multispezies Pellet-Heterogenitäten und deren Auswirkungen auf Produktbildung in der Zellfabrik Aspergillus niger (427889137) |0 G:(GEPRIS)427889137 |c 427889137 |x 3 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)315384307 - Verallgemeinerte morphologische Modellierung aggregierender, filamentöser Mikroorganismen (315384307) |0 G:(GEPRIS)315384307 |c 315384307 |x 4 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)315305620 - Untersuchung des Einflusses von Scherkräften auf das morphogenetische Gennetzwerk, die Zellintegrität, mikroskopische und makroskopische Morphologie von Aspergillus niger sowie Bildungsraten intra- und extrazellulärer Produkte (315305620) |0 G:(GEPRIS)315305620 |c 315305620 |x 5 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)425860318 - Koordination des DFG-Schwerpunktprogramms 1934 DiSPBiotech (425860318) |0 G:(GEPRIS)425860318 |c 425860318 |x 6 |
| 542 | _ | _ | |i 2024-06-29 |2 Crossref |u http://creativecommons.org/licenses/by-nc/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: bib-pubdb1.desy.de |
| 693 | _ | _ | |a PETRA III |f PETRA Beamline P05 |1 EXP:(DE-H253)PETRAIII-20150101 |0 EXP:(DE-H253)P-P05-20150101 |6 EXP:(DE-H253)P-P05-20150101 |x 0 |
| 700 | 1 | _ | |a Müller, Henri |0 P:(DE-H253)PIP1092635 |b 1 |
| 700 | 1 | _ | |a Kellhammer, Diana |0 0009-0009-4883-5027 |b 2 |
| 700 | 1 | _ | |a Deffur, Charlotte |0 P:(DE-H253)PIP1100576 |b 3 |
| 700 | 1 | _ | |a Schmideder, Stefan |0 P:(DE-H253)PIP1092641 |b 4 |
| 700 | 1 | _ | |a Hammel, Jörg U. |0 P:(DE-H253)PIP1008646 |b 5 |
| 700 | 1 | _ | |a Krull, Rainer |0 0000-0003-2821-8610 |b 6 |
| 700 | 1 | _ | |a Briesen, Heiko |0 P:(DE-H253)PIP1092638 |b 7 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1002/bit.28788 |b Wiley |d 2024-06-29 |n 10 |p 3128-3143 |3 journal-article |2 Crossref |t Biotechnology and Bioengineering |v 121 |y 2024 |x 0006-3592 |
| 773 | _ | _ | |a 10.1002/bit.28788 |g p. bit.28788 |0 PERI:(DE-600)280319-7 |n 10 |p 3128-3143 |t Biotechnology and Bioengineering / Symposium |v 121 |y 2024 |x 0006-3592 |
| 856 | 4 | _ | |y OpenAccess |u https://bib-pubdb1.desy.de/record/611461/files/Biotech%20Bioengineering%20-%202024%20-%20Dinius%20-%203D%20imaging%20and%20analysis%20to%20unveil%20the%20impact%20of%20microparticles%20on%20the%20pellet.pdf |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://bib-pubdb1.desy.de/record/611461/files/Biotech%20Bioengineering%20-%202024%20-%20Dinius%20-%203D%20imaging%20and%20analysis%20to%20unveil%20the%20impact%20of%20microparticles%20on%20the%20pellet.pdf?subformat=pdfa |
| 909 | C | O | |o oai:bib-pubdb1.desy.de:611461 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 0 |6 P:(DE-H253)PIP1100470 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 1 |6 P:(DE-H253)PIP1092635 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 3 |6 P:(DE-H253)PIP1100576 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 4 |6 P:(DE-H253)PIP1092641 |
| 910 | 1 | _ | |a Helmholtz-Zentrum Geesthacht |0 I:(DE-588b)16087541-9 |k HZG |b 5 |6 P:(DE-H253)PIP1008646 |
| 910 | 1 | _ | |a Helmholtz-Zentrum Hereon |0 I:(DE-588b)1231250402 |k Hereon |b 5 |6 P:(DE-H253)PIP1008646 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 7 |6 P:(DE-H253)PIP1092638 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Großgeräte: Materie |1 G:(DE-HGF)POF4-6G0 |0 G:(DE-HGF)POF4-6G3 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-600 |4 G:(DE-HGF)POF |v PETRA III (DESY) |x 0 |
| 914 | 1 | _ | |y 2024 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2023-04-25 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b BIOTECHNOL BIOENG : 2021 |d 2023-04-25 |
| 915 | _ | _ | |a Creative Commons Attribution-NonCommercial CC BY-NC 4.0 |0 LIC:(DE-HGF)CCBYNC4 |2 HGFVOC |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2023-04-25 |
| 915 | _ | _ | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID |d 2023-04-25 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2023-04-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2023-04-25 |
| 920 | 1 | _ | |0 I:(DE-H253)HAS-User-20120731 |k DOOR ; HAS-User |l DOOR-User |x 0 |
| 920 | 1 | _ | |0 I:(DE-H253)Hereon-20210428 |k Hereon |l Helmholtz-Zentrum Hereon |x 1 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-H253)HAS-User-20120731 |
| 980 | _ | _ | |a I:(DE-H253)Hereon-20210428 |
| 980 | 1 | _ | |a FullTexts |
| 999 | C | 5 | |a 10.1016/j.ultramic.2015.05.002 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1364/OE.24.025129 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s11274-016-2148-7 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s10529-015-2019-6 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s11274-009-0140-1 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/elsc.202000060 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/s40694-018-0054-5 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/s13068-019-1400-4 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/2191-0855-3-27 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1515/psr-2022-0112 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.3389/fbioe.2023.1171055 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.23313 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s00253-010-2661-9 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.22614 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s13205-020-2068-y |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/yea.3022 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/s40694-021-00131-6 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/elsc.201300055 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |1 Greving I. |y 2014 |2 Crossref |t SPIE proceedings, developments in X‐ray tomography IX |o Greving I. SPIE proceedings, developments in X‐ray tomography IX 2014 |
| 999 | C | 5 | |a 10.1111/jfpp.14915 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |1 Haibel A. |y 2010 |2 Crossref |t SPIE proceedings, developments in X‐ray tomography |o Haibel A. SPIE proceedings, developments in X‐ray tomography 2010 |
| 999 | C | 5 | |a 10.1002/bit.21713 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/mbo3.603 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.jbiotec.2012.06.024 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.27537 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/elsc.202100075 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.bej.2009.12.016 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/0165-1684(94)90060-4 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1038/nprot.2014.033 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.28506 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1109/TSMC.1979.4310076 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.jsb.2011.07.017 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/1475-2859-5-3 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.fgb.2012.05.003 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.26956 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.27166 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/bit.27622 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1371/journal.pone.0234125 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s00253-018-8818-7 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1063/1.4952858 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.copbio.2019.02.010 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1186/1475-2859-10-58 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/S0065-2164(10)72004-9 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1016/j.fbp.2020.02.003 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1007/s00449-016-1615-8 |9 -- missing cx lookup -- |2 Crossref |
| 999 | C | 5 | |a 10.1002/cbic.200700486 |9 -- missing cx lookup -- |2 Crossref |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|