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@ARTICLE{Dinius:611461,
      author       = {Dinius, Anna and Müller, Henri and Kellhammer, Diana and
                      Deffur, Charlotte and Schmideder, Stefan and Hammel, Jörg
                      U. and Krull, Rainer and Briesen, Heiko},
      title        = {3{D} imaging and analysis to unveil the impact of
                      microparticles on the pellet morphology of filamentous
                      fungi},
      journal      = {Biotechnology and Bioengineering / Symposium},
      volume       = {121},
      number       = {10},
      issn         = {0006-3592},
      address      = {New York, NY},
      publisher    = {Wiley},
      reportid     = {PUBDB-2024-04926},
      pages        = {3128-3143},
      year         = {2024},
      note         = {ISSN 1097-0290 not unique: **2 hits**.},
      abstract     = {Controlling the morphology of filamentous fungi is crucial
                      to improve the performance of fungal bioprocesses.
                      Microparticle-enhanced cultivation (MPEC) increases
                      productivity, most likely by changing the fungal morphology.
                      However, due to a lack of appropriate methods, the exact
                      impact of the added microparticles on the structural
                      development of fungal pellets is mostly unexplored. In this
                      study synchrotron radiation-based microcomputed tomography
                      and three-dimensional (3D) image analysis were applied to
                      unveil the detailed 3D incorporation of glass microparticles
                      in nondestructed pellets of Aspergillus niger from MPEC. The
                      developed method enabled the 3D analysis based on 375
                      pellets from various MPEC experiments. The total and locally
                      resolved volume fractions of glass microparticles and hyphae
                      were quantified for the first time. At increasing
                      microparticle concentrations in the culture medium, pellets
                      with lower hyphal fraction were obtained. However, the total
                      volume of incorporated glass microparticles within the
                      pellets did not necessarily increase. Furthermore, larger
                      microparticles were less effective than smaller ones in
                      reducing pellet density. However, the total volume of
                      incorporated glass was larger for large microparticles. In
                      addition, analysis of MPEC pellets from different times of
                      cultivation indicated that spore agglomeration is decisive
                      for the development of MPEC pellets. The developed 3D
                      morphometric analysis method and the presented results will
                      promote the general understanding and further development of
                      MPEC for industrial application.},
      cin          = {DOOR ; HAS-User / Hereon},
      ddc          = {570},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)Hereon-20210428},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20211150
                      (I-20211150) / FS-Proposal: I-20200214 (I-20200214) / DFG
                      project G:(GEPRIS)427889137 - Kontrolle von Monospezies und
                      Multispezies Pellet-Heterogenitäten und deren Auswirkungen
                      auf Produktbildung in der Zellfabrik Aspergillus niger
                      (427889137) / DFG project G:(GEPRIS)315384307 -
                      Verallgemeinerte morphologische Modellierung aggregierender,
                      filamentöser Mikroorganismen (315384307) / DFG project
                      G:(GEPRIS)315305620 - Untersuchung des Einflusses von
                      Scherkräften auf das morphogenetische Gennetzwerk, die
                      Zellintegrität, mikroskopische und makroskopische
                      Morphologie von Aspergillus niger sowie Bildungsraten intra-
                      und extrazellulärer Produkte (315305620) / DFG project
                      G:(GEPRIS)425860318 - Koordination des
                      DFG-Schwerpunktprogramms 1934 DiSPBiotech (425860318)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20211150 /
                      G:(DE-H253)I-20200214 / G:(GEPRIS)427889137 /
                      G:(GEPRIS)315384307 / G:(GEPRIS)315305620 /
                      G:(GEPRIS)425860318},
      experiment   = {EXP:(DE-H253)P-P05-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38943490},
      UT           = {WOS:001258645700001},
      doi          = {10.1002/bit.28788},
      url          = {https://bib-pubdb1.desy.de/record/611461},
}