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000611461 1001_ $$0P:(DE-H253)PIP1100470$$aDinius, Anna$$b0
000611461 245__ $$a3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi
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000611461 520__ $$aControlling the morphology of filamentous fungi is crucial to improve the performance of fungal bioprocesses. Microparticle-enhanced cultivation (MPEC) increases productivity, most likely by changing the fungal morphology. However, due to a lack of appropriate methods, the exact impact of the added microparticles on the structural development of fungal pellets is mostly unexplored. In this study synchrotron radiation-based microcomputed tomography and three-dimensional (3D) image analysis were applied to unveil the detailed 3D incorporation of glass microparticles in nondestructed pellets of Aspergillus niger from MPEC. The developed method enabled the 3D analysis based on 375 pellets from various MPEC experiments. The total and locally resolved volume fractions of glass microparticles and hyphae were quantified for the first time. At increasing microparticle concentrations in the culture medium, pellets with lower hyphal fraction were obtained. However, the total volume of incorporated glass microparticles within the pellets did not necessarily increase. Furthermore, larger microparticles were less effective than smaller ones in reducing pellet density. However, the total volume of incorporated glass was larger for large microparticles. In addition, analysis of MPEC pellets from different times of cultivation indicated that spore agglomeration is decisive for the development of MPEC pellets. The developed 3D morphometric analysis method and the presented results will promote the general understanding and further development of MPEC for industrial application. 
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000611461 536__ $$0G:(GEPRIS)427889137$$aDFG project G:(GEPRIS)427889137 - Kontrolle von Monospezies und Multispezies Pellet-Heterogenitäten und deren Auswirkungen auf Produktbildung in der Zellfabrik Aspergillus niger (427889137)$$c427889137$$x3
000611461 536__ $$0G:(GEPRIS)315384307$$aDFG project G:(GEPRIS)315384307 - Verallgemeinerte morphologische Modellierung aggregierender, filamentöser Mikroorganismen (315384307)$$c315384307$$x4
000611461 536__ $$0G:(GEPRIS)315305620$$aDFG project G:(GEPRIS)315305620 - Untersuchung des Einflusses von Scherkräften auf das morphogenetische Gennetzwerk, die Zellintegrität, mikroskopische und makroskopische Morphologie von Aspergillus niger sowie Bildungsraten intra- und extrazellulärer Produkte (315305620)$$c315305620$$x5
000611461 536__ $$0G:(GEPRIS)425860318$$aDFG project G:(GEPRIS)425860318 - Koordination des DFG-Schwerpunktprogramms 1934 DiSPBiotech (425860318)$$c425860318$$x6
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000611461 7001_ $$0P:(DE-H253)PIP1092635$$aMüller, Henri$$b1
000611461 7001_ $$00009-0009-4883-5027$$aKellhammer, Diana$$b2
000611461 7001_ $$0P:(DE-H253)PIP1100576$$aDeffur, Charlotte$$b3
000611461 7001_ $$0P:(DE-H253)PIP1092641$$aSchmideder, Stefan$$b4
000611461 7001_ $$0P:(DE-H253)PIP1008646$$aHammel, Jörg U.$$b5
000611461 7001_ $$00000-0003-2821-8610$$aKrull, Rainer$$b6
000611461 7001_ $$0P:(DE-H253)PIP1092638$$aBriesen, Heiko$$b7$$eCorresponding author
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