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| Home > Publications database > Crystal structure of the tegument protein UL82 (pp71) from human cytomegalovirus > print |
| 001 | 605887 | ||
| 005 | 20250723171714.0 | ||
| 024 | 7 | _ | |a 10.1002/pro.4915 |2 doi |
| 024 | 7 | _ | |a 0961-8368 |2 ISSN |
| 024 | 7 | _ | |a 1469-896X |2 ISSN |
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| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Eberhage, Jan |b 0 |
| 245 | _ | _ | |a Crystal structure of the tegument protein UL82 (pp71) from human cytomegalovirus |
| 260 | _ | _ | |a Bethesda, Md. |c 2024 |b Protein Society |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1727095264_1802682 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Human cytomegalovirus (HCMV) is an opportunistic pathogen that infects a majority of the world population. It may cause severe disease in immunocompromised people and lead to pregnancy loss or grave disabilities of the fetus upon congenital infection. For effective replication and lifelong persistence in its host, HCMV relies on diverse functions of its tegument protein UL82, also known as pp71. Up to now, little is known about the molecular mechanisms underlying the multiple functions of this crucial viral protein. Here, we describe the X-ray structure of full-length UL82 to a resolution of 2.7 Å. A single polypeptide chain of 559 amino acids mainly folds into three ß-barrels. We show that UL82 forms a dimer in the crystal as well as in solution. We identify point mutations that disturb the dimerization interface and show that the mutant protein is monomeric in solution and upon expression in human cells. On the basis of the three-dimensional structure, we identify structural homologs of UL82 from other herpesviruses and analyze whether their functions are preserved in UL82. We demonstrate that UL82, despite its structural homology to viral deoxyuridinetriphosphatases (dUTPases), does not possess dUTPase activity. Prompted by the structural homology of UL82 to the ORF10 protein of murine herpesvirus 68 (MHV68), which is known to interact with the RNA export factor ribonucleic acid export 1 (Rae1), we performed coimmunoprecipitations and demonstrated that UL82 indeed interacts with Rae1. This suggests that HCMV UL82 may play a role in mRNA export from the nucleus similar to ORF10 encoded by the gammaherpesviruses MHV68. |
| 536 | _ | _ | |a 6G3 - PETRA III (DESY) (POF4-6G3) |0 G:(DE-HGF)POF4-6G3 |c POF4-6G3 |f POF IV |x 0 |
| 536 | _ | _ | |a DFG project 390874280 - EXC 2155: RESIST - Resolving Infection Susceptibility (390874280) |0 G:(GEPRIS)390874280 |c 390874280 |x 1 |
| 542 | _ | _ | |i 2024-02-15 |2 Crossref |u http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: bib-pubdb1.desy.de |
| 693 | _ | _ | |a PETRA III |f PETRA Beamline P13 |1 EXP:(DE-H253)PETRAIII-20150101 |0 EXP:(DE-H253)P-P13-20150101 |6 EXP:(DE-H253)P-P13-20150101 |x 0 |
| 700 | 1 | _ | |a Bresch, Ian P. |b 1 |
| 700 | 1 | _ | |a Ramani, Ramya |b 2 |
| 700 | 1 | _ | |a Viohl, Niklas |b 3 |
| 700 | 1 | _ | |a Buchta, Thalea |b 4 |
| 700 | 1 | _ | |a Rehfeld, Christopher L. |b 5 |
| 700 | 1 | _ | |a Hinse, Petra |b 6 |
| 700 | 1 | _ | |a Reubold, Thomas F. |b 7 |
| 700 | 1 | _ | |a Brinkmann, Melanie M. |0 P:(DE-HGF)0 |b 8 |e Corresponding author |
| 700 | 1 | _ | |a Eschenburg, Susanne |0 0000-0002-7422-618X |b 9 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1002/pro.4915 |b Wiley |d 2024-02-15 |n 3 |3 journal-article |2 Crossref |t Protein Science |v 33 |y 2024 |x 0961-8368 |
| 773 | _ | _ | |a 10.1002/pro.4915 |g Vol. 33, no. 3, p. e4915 |0 PERI:(DE-600)2000025-X |n 3 |p e4915 |t Protein science |v 33 |y 2024 |x 0961-8368 |
| 856 | 4 | _ | |u https://onlinelibrary.wiley.com/doi/full/10.1002/pro.4915 |
| 856 | 4 | _ | |u https://bib-pubdb1.desy.de/record/605887/files/Crystal%20structure%20of%20the%20tegument%20protein%20UL82%20pp71%20from%20human%20cytomegalovirus.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://bib-pubdb1.desy.de/record/605887/files/Crystal%20structure%20of%20the%20tegument%20protein%20UL82%20pp71%20from%20human%20cytomegalovirus.pdf?subformat=pdfa |x pdfa |y OpenAccess |
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| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Großgeräte: Materie |1 G:(DE-HGF)POF4-6G0 |0 G:(DE-HGF)POF4-6G3 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-600 |4 G:(DE-HGF)POF |v PETRA III (DESY) |x 0 |
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