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@ARTICLE{Henkel:603124,
author = {Henkel, A. and Maracke, J. and Munke, A. and Galchenkova,
M. and Rahmani Mashhour, A. and Reinke, P. and Domaracky,
Martin and Fleckenstein, Holger and Hakanpää, J. and
Meyer, Jan and Tolstikova, A. and Carnis, J. and Middendorf,
P. and Gelisio, L. and Yefanov, O. and Chapman, H. N. and
Oberthür, D.},
title = {{CFEL} {T}ape{D}rive 2.0: a conveyor belt-based
sample-delivery system for multi-dimensional serial
crystallography},
journal = {Acta crystallographica / Section A},
volume = {78},
number = {a2},
issn = {0108-7673},
address = {Oxford [u.a.]},
publisher = {Blackwell},
reportid = {PUBDB-2024-00769},
pages = {e560},
year = {2022},
abstract = {Serial crystallography (SX) at both X-ray free-electron
lasers (XFELs) and synchrotrons offers the possibility to
collect data at non-cryogenic temperature almost radiation
damage free and enables time-resolved crystallography of
irreversible reactions. The necessary steady delivery of new
micron-sized crystals of biological macromolecules sets it
apart from traditional single crystal macromolecular
crystallography (MX). For this, many new means of sample
delivery have been developed [1]. Described here is a novel
conveyor belt-based sample delivery system, the
completelyre-designed and reengineered second generation of
the CFEL TapeDrive. It is optimized for fast installation at
beamlines, ease of use, low sample consumption and precise
adjustment of several sample delivery parameters, like
ligand concentration, pH and sample temperature. Through
combination of these parameters and the additional
possibility for time-resolved experiments (delay times of 50
ms – 180 s), CFEL TapeDrive 2.0 enables multidimensional
serial crystallography experiments. It can now be used as
standard instrumentation at beamline P11 at PETRA III (DESY,
Germany) and is suitable for serial Laue crystallography
with polychromatic X-rays. Additionally, it can be used at
XFELs in air (e.g. at SwissFEL and LCLS), offering the
possibility to combine the advantages of jet-based injection
with those of fixed targetbased systems. In the future,
parts of the system will be automated to achieve rapid
ligand screening with micron-sized crystals in various
sample environments. By introducing the CFEL TapeDrive 2.0,
a platform for user-friendly multi-dimensional serial
crystallography, we provide a novel approach for structural
biology to further reveal details about how macromolecules
keep our biological world turning.},
cin = {FS-PETRA-D / $FS_FS-SC$ / CFEL-I / FS-CFEL-1-BMX},
ddc = {530},
cid = {I:(DE-H253)FS-PETRA-D-20210408 /
$I:(DE-H253)FS_FS-SC-20201126$ / I:(DE-H253)CFEL-I-20161114
/ I:(DE-H253)FS-CFEL-1-BMX-20210408},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P11-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:001048207700468},
doi = {10.1107/S2053273322092038},
url = {https://bib-pubdb1.desy.de/record/603124},
}