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000602551 1001_ $$0P:(DE-H253)PIP1098893$$aWilliams, Harry$$b0
000602551 245__ $$aStructural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein
000602551 260__ $$aOxford$$bOxford Univ. Press$$c2023
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000602551 500__ $$apart of this work was performed at the Cryo-EM multi-user Facility at CSSB, headed by K.G. and sup-ported by the UHH and DFG [INST 152/772-1, 774-1, 775-1, 776-1];
000602551 520__ $$aSevere fever with thrombocytopenia syndrome virus (SFTSV) is a phenuivirus that has rapidly become endemic in several East Asian countries. The large (L) protein of SFTSV, which includes the RNA-dependent RNA polymerase (RdRp), is responsible for catalysing viral genome replication and transcription. Here, we present 5 cryo-electron microscopy (cryo-EM) structures of the L protein in several states of the genome replication process, from pre-initiation to late-stage elongation, at a resolution of up to 2.6 Å. We identify how the L protein binds the 5′ viral RNA in a hook-like conformation and show how the distal 5′ and 3′ RNA ends form a duplex positioning the 3′ RNA terminus in the RdRp active site ready for initiation. We also observe the L protein stalled in the early and late stages of elongation with the RdRp core accommodating a 10-bp product-template duplex. This duplex ultimately splits with the template binding to a designated 3′ secondary binding site. The structural data and observations are complemented by in vitro biochemical and cell-based mini-replicon assays. Altogether, our data provide novel key insights into the mechanism of viral genome replication by the SFTSV L protein and will aid drug development against segmented negative-strand RNA viruses.
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000602551 7001_ $$0P:(DE-H253)PIP1088744$$aThorkelsson, Sigurdur R$$b1
000602551 7001_ $$0P:(DE-H253)PIP1025990$$aVogel, Dominik$$b2
000602551 7001_ $$0P:(DE-H253)PIP1014518$$aMilewski, Morlin$$b3
000602551 7001_ $$0P:(DE-HGF)0$$aBusch, Carola$$b4
000602551 7001_ $$0P:(DE-HGF)0$$aCusack, Stephen$$b5
000602551 7001_ $$0P:(DE-H253)PIP1023782$$aGruenewald, Kay$$b6$$udesy
000602551 7001_ $$0P:(DE-H253)PIP1082957$$aQuemin, Emmanuelle$$b7
000602551 7001_ $$0P:(DE-HGF)0$$aRosenthal, Maria$$b8$$eCorresponding author
000602551 773__ $$0PERI:(DE-600)2205588-5$$a10.1093/nar/gkac1249$$gVol. 51, no. 3, p. 1424 - 1442$$n3$$p1424 - 1442$$tNucleic acids symposium series$$v51$$x0305-1048$$y2023
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