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@ARTICLE{Srivastava:602314,
      author       = {Srivastava, Sukrit and Verma, Sonia and Kamthania, Mohit
                      and Agarwal, Deepa and Saxena, Ajay Kumar and Kolbe, Michael
                      and Singh, Sarman and Kotnis, Ashwin and Rathi, Brijesh and
                      Nayar, Seema A. and Shin, Ho-Joon and Vashisht, Kapil and
                      Pandey, Kailash C.},
      title        = {{C}omputationally validated {SARS}-{C}o{V}-2 {CTL} and
                      {HTL} {M}ulti-{P}atch vaccines, designed by reverse
                      epitomics approach, show potential to cover large ethnically
                      distributed human population worldwide},
      journal      = {Journal of biomolecular structure $\&$ dynamics},
      volume       = {40},
      number       = {5},
      issn         = {0739-1102},
      address      = {Abingdon [u.a.]},
      publisher    = {Taylor $\&$ Francis},
      reportid     = {PUBDB-2024-00589},
      pages        = {2369 - 2388},
      year         = {2022},
      abstract     = {The SARS-CoV-2 (Severe Acute Respiratory Syndrome
                      Coronavirus 2) is responsible for the COVID-19 outbreak. The
                      highly contagious COVID-19 disease has spread to 216
                      countries in less than six months. Though several vaccine
                      candidates are being claimed, an effective vaccine is yet to
                      come. A novel reverse epitomics approach,
                      ‘overlapping-epitope-clusters-to-patches’ method is
                      utilized to identify the antigenic regions from the
                      SARS-CoV-2 proteome. These antigenic regions are named as
                      ‘Ag-Patch or Ag-Patches’, for Antigenic Patch or
                      Patches. The identification of Ag-Patches is based on the
                      clusters of overlapping epitopes rising from SARS-CoV-2
                      proteins. Further, we have utilized the identified
                      Ag-Patches to design Multi-Patch Vaccines (MPVs), proposing
                      a novel method for the vaccine design. The designed MPVs
                      were analyzed for immunologically crucial parameters,
                      physiochemical properties and cDNA constructs. We identified
                      73 CTL (Cytotoxic T-Lymphocyte) and 49 HTL (Helper
                      T-Lymphocyte) novel Ag-Patches from the proteome of
                      SARS-CoV-2. The identified Ag-Patches utilized to design
                      MPVs cover 768 overlapping epitopes targeting 55 different
                      HLA alleles leading to $99.98\%$ of world human population
                      coverage. The MPVs and Toll-Like Receptor ectodomain complex
                      shows stable complex formation tendency. Further, the cDNA
                      analysis favors high expression of the MPVs constructs in a
                      human cell line. We identified highly immunogenic novel
                      Ag-Patches from the entire proteome of SARS CoV-2 by a novel
                      reverse epitomics approach and utilized them to design MPVs.
                      We conclude that the novel MPVs could be a highly potential
                      novel approach to combat SARS-CoV-2, with greater
                      effectiveness, high specificity and large human population
                      coverage worldwide.},
      cin          = {CSSB-HZI-MK},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-HZI-MK-20210520},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33155524},
      UT           = {WOS:000586073100001},
      doi          = {10.1080/07391102.2020.1838329},
      url          = {https://bib-pubdb1.desy.de/record/602314},
}