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024 7 _ |a 10.3390/bios11030060
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100 1 _ |a Stinn, Anne
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245 _ _ |a Novel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis
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520 _ _ |a The aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor of a variety of environmental pollutants and dietary-, cell- and microbiota-derived metabolites with important roles in fundamental biological processes. Deregulation of the AhR pathway is implicated in several diseases, including autoimmune diseases and cancer, rendering AhR a promising target for drug development and host-directed therapy. The pharmacological intervention of AhR processes requires detailed information about the ligand binding properties to allow specific targeting of a particular signaling process without affecting the remaining. Here, we present a novel microscale thermophoresis-based approach to monitoring the binding of purified recombinant human AhR to its natural ligands in a cell-free system. This approach facilitates a precise identification and characterization of unknown AhR ligands and represents a screening strategy for the discovery of potential selective AhR modulators.
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700 1 _ |a Furkert, Jens
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700 1 _ |a Kaufmann, Stefan H. E.
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700 1 _ |a Moura-Alves, Pedro
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700 1 _ |a Kolbe, Michael
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773 _ _ |a 10.3390/bios11030060
|g Vol. 11, no. 3, p. 60 -
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|t Biosensors
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