TY  - JOUR
AU  - Stinn, Anne
AU  - Furkert, Jens
AU  - Kaufmann, Stefan H. E.
AU  - Moura-Alves, Pedro
AU  - Kolbe, Michael
TI  - Novel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis
JO  - Biosensors
VL  - 11
IS  - 3
SN  - 2079-6374
CY  - Basel
PB  - MDPI
M1  - PUBDB-2024-00588
SP  - 60 
PY  - 2021
AB  - The aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor of a variety of environmental pollutants and dietary-, cell- and microbiota-derived metabolites with important roles in fundamental biological processes. Deregulation of the AhR pathway is implicated in several diseases, including autoimmune diseases and cancer, rendering AhR a promising target for drug development and host-directed therapy. The pharmacological intervention of AhR processes requires detailed information about the ligand binding properties to allow specific targeting of a particular signaling process without affecting the remaining. Here, we present a novel microscale thermophoresis-based approach to monitoring the binding of purified recombinant human AhR to its natural ligands in a cell-free system. This approach facilitates a precise identification and characterization of unknown AhR ligands and represents a screening strategy for the discovery of potential selective AhR modulators. 
LB  - PUB:(DE-HGF)16
C6  - 33668313
UR  - <Go to ISI:>//WOS:000633434900001
DO  - DOI:10.3390/bios11030060
UR  - https://bib-pubdb1.desy.de/record/602313
ER  -