%0 Journal Article
%A Stinn, Anne
%A Furkert, Jens
%A Kaufmann, Stefan H. E.
%A Moura-Alves, Pedro
%A Kolbe, Michael
%T Novel Method for Quantifying AhR-Ligand Binding Affinities Using Microscale Thermophoresis
%J Biosensors
%V 11
%N 3
%@ 2079-6374
%C Basel
%I MDPI
%M PUBDB-2024-00588
%P 60 
%D 2021
%X The aryl hydrocarbon receptor (AhR) is a highly conserved cellular sensor of a variety of environmental pollutants and dietary-, cell- and microbiota-derived metabolites with important roles in fundamental biological processes. Deregulation of the AhR pathway is implicated in several diseases, including autoimmune diseases and cancer, rendering AhR a promising target for drug development and host-directed therapy. The pharmacological intervention of AhR processes requires detailed information about the ligand binding properties to allow specific targeting of a particular signaling process without affecting the remaining. Here, we present a novel microscale thermophoresis-based approach to monitoring the binding of purified recombinant human AhR to its natural ligands in a cell-free system. This approach facilitates a precise identification and characterization of unknown AhR ligands and represents a screening strategy for the discovery of potential selective AhR modulators. 
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 33668313
%U <Go to ISI:>//WOS:000633434900001
%R 10.3390/bios11030060
%U https://bib-pubdb1.desy.de/record/602313