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| 001 | 602312 | ||
| 005 | 20250716150758.0 | ||
| 024 | 7 | _ | |a 10.1016/j.bbrep.2021.101039 |2 doi |
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| 245 | _ | _ | |a Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2021 |b Elsevier |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 500 | _ | _ | |a Part of this work was performed at the EM facility at CSSB, supported by UHH and DFG grant numbers (INST 152/772–1|152/774–1|152/775–1|152/776–1|152-777-1 FUGG). |
| 520 | _ | _ | |a Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development. |
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| 536 | _ | _ | |a FUTURE T3SS - Bacterial effector secretion: Function and Architecture of the Type 3 Secretion System (311374) |0 G:(EU-Grant)311374 |c 311374 |f ERC-2012-StG_20111109 |x 1 |
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| 700 | 1 | _ | |a Lunelli, Michele |0 P:(DE-H253)PIP1021127 |b 1 |
| 700 | 1 | _ | |a Wald, Jiri |0 P:(DE-H253)PIP1083333 |b 2 |
| 700 | 1 | _ | |a Kolbe, Michael |0 P:(DE-H253)PIP1021134 |b 3 |e Corresponding author |
| 700 | 1 | _ | |a Marlovits, Thomas |0 P:(DE-H253)PIP1021412 |b 4 |e Corresponding author |
| 773 | _ | _ | |a 10.1016/j.bbrep.2021.101039 |g Vol. 27, p. 101039 - |0 PERI:(DE-600)2831046-9 |p 101039 |t Biochemistry and biophysics reports |v 27 |y 2021 |x 2405-5808 |
| 856 | 4 | _ | |y OpenAccess |u https://bib-pubdb1.desy.de/record/602312/files/1-s2.0-S2405580821001333-main.pdf |
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