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@ARTICLE{Kotov:602312,
      author       = {Kotov, Vadim and Lunelli, Michele and Wald, Jiri and Kolbe,
                      Michael and Marlovits, Thomas},
      title        = {{H}elical reconstruction of {S}almonella and {S}higella
                      needle filaments attached to type 3 basal bodies},
      journal      = {Biochemistry and biophysics reports},
      volume       = {27},
      issn         = {2405-5808},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {PUBDB-2024-00587},
      pages        = {101039},
      year         = {2021},
      note         = {Part of this work was performed at the EM facility at CSSB,
                      supported by UHH and DFG grant numbers (INST
                      152/772–1|152/774–1|152/775–1|152/776–1|152-777-1
                      FUGG).},
      abstract     = {Gram-negative pathogens evolved a syringe-like nanomachine,
                      termed type 3 secretion system, to deliver protein effectors
                      into the cytoplasm of host cells. An essential component of
                      this system is a long helical needle filament that protrudes
                      from the bacterial surface and connects the cytoplasms of
                      the bacterium and the eukaryotic cell. Previous structural
                      research was predominantly focused on reconstituted type 3
                      needle filaments, which lacked the biological context. In
                      this work we introduce a facile procedure to obtain
                      high-resolution cryo-EM structure of needle filaments
                      attached to the basal body of type 3 secretion systems. We
                      validate our approach by solving the structure of Salmonella
                      PrgI filament and demonstrate its utility by obtaining the
                      first high-resolution cryo-EM reconstruction of Shigella
                      MxiH filament. Our work paves the way to systematic
                      structural characterization of attached type 3 needle
                      filaments in the context of mutagenesis studies, protein
                      structural evolution and drug development.},
      cin          = {CSSB-HZI-MK / CSSB-UKE-TM},
      ddc          = {530},
      cid          = {I:(DE-H253)CSSB-HZI-MK-20210520 /
                      I:(DE-H253)CSSB-UKE-TM-20210520},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / FUTURE T3SS - Bacterial effector
                      secretion: Function and Architecture of the Type 3 Secretion
                      System (311374) / iNEXT - Infrastructure for NMR, EM and
                      X-rays for translational research (653706)},
      pid          = {G:(DE-HGF)POF4-633 / G:(EU-Grant)311374 /
                      G:(EU-Grant)653706},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34258394},
      UT           = {WOS:000701241600009},
      doi          = {10.1016/j.bbrep.2021.101039},
      url          = {https://bib-pubdb1.desy.de/record/602312},
}