TY  - JOUR
AU  - Kotov, Vadim
AU  - Lunelli, Michele
AU  - Wald, Jiri
AU  - Kolbe, Michael
AU  - Marlovits, Thomas
TI  - Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies
JO  - Biochemistry and biophysics reports
VL  - 27
SN  - 2405-5808
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PUBDB-2024-00587
SP  - 101039 
PY  - 2021
N1  - Part of this work was performed at the EM facility at CSSB, supported by UHH and DFG grant numbers (INST 152/772–1|152/774–1|152/775–1|152/776–1|152-777-1 FUGG).
AB  - Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development. 
LB  - PUB:(DE-HGF)16
C6  - pmid:34258394
UR  - <Go to ISI:>//WOS:000701241600009
DO  - DOI:10.1016/j.bbrep.2021.101039
UR  - https://bib-pubdb1.desy.de/record/602312
ER  -