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@ARTICLE{Zhang:601932,
author = {Zhang, Chi and Parichenko, Alexandra and Choi, Wonyeong and
Shin, Seonghwan and Panes-Ruiz, Luis Antonio and Belyaev,
Dmitry and Custódio, Tânia Filipa and Löw, Christian and
Lee, Jeong-Soo and Ibarlucea, Bergoi and Cuniberti,
Gianaurelio},
title = {{S}ybodies as {N}ovel {B}ioreceptors toward
{F}ield-{E}ffect {T}ransistor-{B}ased {D}etection of
{SARS}-{C}o{V}-2 {A}ntigens},
journal = {ACS applied materials $\&$ interfaces},
volume = {15},
number = {34},
issn = {1944-8244},
address = {Washington, DC},
publisher = {Soc.},
reportid = {PUBDB-2024-00422},
pages = {40191 - 40200},
year = {2023},
abstract = {The SARS-CoV-2 pandemic has increased the demand for
low-cost, portable, and rapid biosensors, driving huge
research efforts toward new nanomaterial-based approaches
with high sensitivity. Many of them employ antibodies as
bioreceptors, which have a costly development process that
requires animal facilities. Recently, sybodies emerged as a
new alternative class of synthetic binders and receptors
with high antigen binding efficiency, improved chemical
stability, and lower production costs via animal-free
methods. Their smaller size is an important asset to
consider in combination with ultrasensitive field-effect
transistors (FETs) as transducers, which respond more
intensely when biorecognition occurs near their surface.
This work demonstrates the immobilization of sybodies
against the spike protein of the virus on silicon surfaces,
which are often integral parts of the semiconducting channel
of FETs. Immobilized sybodies maintain the capability to
capture antigens, even at low concentrations in the
femtomolar range, as observed by fluorescence microscopy.
Finally, the first proof of concept of sybody-modified FET
sensing is provided using a nanoscopic silicon net as the
sensitive area where the sybodies are immobilized. The
future development of further sybodies against other
biomarkers and their generalization in biosensors could be
critical to decrease the cost of biodetection platforms in
future pandemics.},
cin = {CSSB-EMBL-CL},
ddc = {600},
cid = {I:(DE-H253)CSSB-EMBL-CL-20210806},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37603713},
UT = {WOS:001052038900001},
doi = {10.1021/acsami.3c06073},
url = {https://bib-pubdb1.desy.de/record/601932},
}