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@ARTICLE{Caragliano:601650,
      author       = {Caragliano, Enrico and Bonazza, Stefano and Frascaroli,
                      Giada and Tang, Jiajia and Soh, Timothy K. and Grünewald,
                      Kay and Bosse, Jens Bernhard and Brune, Wolfram},
      title        = {{H}uman cytomegalovirus forms phase-separated compartments
                      at viral genomes to facilitate viral replication},
      journal      = {Cell reports},
      volume       = {38},
      number       = {10},
      issn         = {2211-1247},
      address      = {[New York, NY]},
      publisher    = {Elsevier},
      reportid     = {PUBDB-2024-00343},
      pages        = {110469},
      year         = {2022},
      abstract     = {SummaryHuman cytomegalovirus (HCMV) replicates its DNA
                      genome in specialized replication compartments (RCs) in the
                      host cell nucleus. These membrane-less organelles originate
                      as spherical structures and grow in size over time. However,
                      the mechanism of RC biogenesis has remained understudied.
                      Using live-cell imaging and photo-oligomerization, we show
                      that a central component of RCs, the UL112-113 proteins,
                      undergo liquid-liquid phase separation (LLPS) to form RCs in
                      the nucleus. We show that the self-interacting domain and
                      large intrinsically disordered regions of UL112-113 are
                      required for LLPS. Importantly, viral DNA induces local
                      clustering of these proteins and lowers the threshold for
                      phase separation. The formation of phase-separated
                      compartments around viral genomes is necessary to recruit
                      the viral DNA polymerase for viral genome replication. Thus,
                      HCMV uses its UL112-113 proteins to generate RCs around
                      viral genomes by LLPS to ensure the formation of a
                      pro-replicative environment.},
      cin          = {CSSB-MHH-JB / CSSB-LIV-KG},
      ddc          = {610},
      cid          = {I:(DE-H253)CSSB-MHH-JB-20210520 /
                      I:(DE-H253)CSSB-LIV-KG-20220525},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project 390874280 - EXC
                      2155: RESIST - Resolving Infection Susceptibility
                      (390874280)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)390874280},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35263605},
      UT           = {WOS:000768295500003},
      doi          = {10.1016/j.celrep.2022.110469},
      url          = {https://bib-pubdb1.desy.de/record/601650},
}