TY  - JOUR
AU  - Caragliano, Enrico
AU  - Bonazza, Stefano
AU  - Frascaroli, Giada
AU  - Tang, Jiajia
AU  - Soh, Timothy K.
AU  - Grünewald, Kay
AU  - Bosse, Jens Bernhard
AU  - Brune, Wolfram
TI  - Human cytomegalovirus forms phase-separated compartments at viral genomes to facilitate viral replication
JO  - Cell reports
VL  - 38
IS  - 10
SN  - 2211-1247
CY  - [New York, NY]
PB  - Elsevier
M1  - PUBDB-2024-00343
SP  - 110469 
PY  - 2022
AB  - SummaryHuman cytomegalovirus (HCMV) replicates its DNA genome in specialized replication compartments (RCs) in the host cell nucleus. These membrane-less organelles originate as spherical structures and grow in size over time. However, the mechanism of RC biogenesis has remained understudied. Using live-cell imaging and photo-oligomerization, we show that a central component of RCs, the UL112-113 proteins, undergo liquid-liquid phase separation (LLPS) to form RCs in the nucleus. We show that the self-interacting domain and large intrinsically disordered regions of UL112-113 are required for LLPS. Importantly, viral DNA induces local clustering of these proteins and lowers the threshold for phase separation. The formation of phase-separated compartments around viral genomes is necessary to recruit the viral DNA polymerase for viral genome replication. Thus, HCMV uses its UL112-113 proteins to generate RCs around viral genomes by LLPS to ensure the formation of a pro-replicative environment. 
LB  - PUB:(DE-HGF)16
C6  - pmid:35263605
UR  - <Go to ISI:>//WOS:000768295500003
DO  - DOI:10.1016/j.celrep.2022.110469
UR  - https://bib-pubdb1.desy.de/record/601650
ER  -