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@ARTICLE{Bogdanow:601641,
author = {Bogdanow, Boris and Gruska, Iris and Mühlberg, Lars and
Protze, Jonas and Hohensee, Svea and Vetter, Barbara and
Bosse, Jens B. and Lehmann, Martin and Sadeghi, Mohsen and
Wiebusch, Lüder and Liu, Fan},
title = {{S}patially resolved protein map of intact human
cytomegalovirus virions},
journal = {Nature microbiology},
volume = {8},
number = {9},
issn = {2058-5276},
address = {London},
publisher = {Nature Publishing Group},
reportid = {PUBDB-2024-00334},
pages = {1732 - 1747},
year = {2023},
abstract = {Herpesviruses assemble large enveloped particles that are
difficult to characterize structurally due to their size,
fragility and complex multilayered proteome with partially
amorphous nature. Here we used crosslinking mass
spectrometry and quantitative proteomics to derive a
spatially resolved interactome map of intact human
cytomegalovirus virions. This enabled the de novo allocation
of 32 viral proteins into four spatially resolved virion
layers, each organized by a dominant viral scaffold protein.
The viral protein UL32 engages with all layers in an
N-to-C-terminal radial orientation, bridging nucleocapsid to
viral envelope. We observed the layer-specific incorporation
of 82 host proteins, of which 39 are selectively recruited.
We uncovered how UL32, by recruitment of PP-1 phosphatase,
antagonizes binding to 14-3-3 proteins. This mechanism
assures effective viral biogenesis, suggesting a perturbing
role of UL32-14-3-3 interaction. Finally, we integrated
these data into a coarse-grained model to provide global
insights into the native configuration of virus and host
protein interactions inside herpesvirions.},
cin = {CSSB-MHH-JB},
ddc = {570},
cid = {I:(DE-H253)CSSB-MHH-JB-20210520},
pnm = {899 - ohne Topic (POF4-899) / DFG project 390874280 - EXC
2155: RESIST - Resolving Infection Susceptibility
(390874280) / SFB 958 A04 - Raumzeitliches Modell neuronaler
Signaltransduktion und ihrer Regulation durch
Präsynaptische Membrangerüste (A04) (202189827) / SFB 1114
C03/Erg. - Anomale Michaelis-Menten-Kinetik in
dichtgepackter, heterogener Umgebung (C03/Erg.) (355497160)},
pid = {G:(DE-HGF)POF4-899 / G:(GEPRIS)390874280 /
G:(GEPRIS)202189827 / G:(GEPRIS)355497160},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37550507},
UT = {WOS:001043678400003},
doi = {10.1038/s41564-023-01433-8},
url = {https://bib-pubdb1.desy.de/record/601641},
}