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@ARTICLE{Geiger:601616,
author = {Geiger, Michael and Brown, Chris and Wichers, Jan Stephan
and Strauss, Jan and Lill, Andrés and Thünauer, Roland and
Liffner, Benjamin and Wilcke, Louisa and Lemcke, Sarah and
Heincke, Dorothee and Pazicky, Samuel and Bachmann, Anna and
Loew, Christian and Wilson, Danny and Filarsky, Michael and
Burda, Paul-Christian and Zhang, Kun and Junop, Murray and
Gilberger, Tim},
title = {{S}tructural {I}nsights {I}nto {P}f{ARO} and
{C}haracterization of its {I}nteraction {W}ith {P}f{AIP}},
journal = {Journal of molecular biology},
volume = {432},
number = {4},
issn = {0022-2836},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {PUBDB-2024-00309},
pages = {878 - 896},
year = {2020},
abstract = {Apicomplexan parasites contain rhoptries, which are
specialized secretory organelles that coordinate host cell
invasion. During the process of invasion, rhoptries secrete
their contents to facilitate interaction with, and entry
into, the host cell. Here, we report the crystal structure
of the rhoptry protein Armadillo Repeats-Only (ARO) from the
human malaria parasite, Plasmodium falciparum (PfARO). The
structure of PfARO comprises five tandem Armadillo-like
(ARM) repeats, with adjacent ARM repeats stacked in a
head-to-tail orientation resulting in PfARO adopting an
elongated curved shape. Interestingly, the concave face of
PfARO contains two distinct patches of highly conserved
residues that appear to play an important role in
protein-protein interaction. We functionally characterized
the P. falciparum homolog of ARO interacting protein (PfAIP)
and demonstrate that it localizes to the rhoptries. We show
that conditional mislocalization of PfAIP leads to deficient
red blood cell invasion. Guided by the structure, we
identified mutations of PfARO that lead to mislocalization
of PfAIP. Using proximity-based biotinylation we probe into
PfAIP interacting proteins.},
cin = {CSSB-BNITM-TG / CSSB-EMBL-CL / CSSB-CF-ALFM / CSSB-UHH-MF},
ddc = {610},
cid = {I:(DE-H253)CSSB-BNITM-TG-20210520 /
I:(DE-H253)CSSB-EMBL-CL-20210806 /
I:(DE-H253)CSSB-CF-ALFM-20210629 /
I:(DE-H253)CSSB-UHH-MF-20210701},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31877322},
UT = {WOS:000518867100008},
doi = {10.1016/j.jmb.2019.12.024},
url = {https://bib-pubdb1.desy.de/record/601616},
}