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@ARTICLE{Balbin:601563,
      author       = {Balbin, Juan M. and Heinemann, Gary K. and Yeoh, Lee M. and
                      Gilberger, Tim-Wolf and Armstrong, Mark and Duffy, Michael
                      F. and Gilson, Paul R. and Wilson, Danny},
      title        = {{C}haracterisation of {P}f{CZIF}1 and {P}f{CZIF}2 in
                      {P}lasmodium falciparum asexual stages},
      journal      = {International journal for parasitology},
      volume       = {53},
      number       = {1},
      issn         = {0020-7519},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {PUBDB-2024-00272},
      pages        = {27 - 41},
      year         = {2023},
      abstract     = {Plasmodium falciparum exerts strong temporal control of
                      gene expression across its lifecycle. Proteins expressed
                      exclusively during late schizogony of blood stages, for
                      example, often have a role in facilitating merozoite
                      invasion of the host red blood cell (RBC), through merozoite
                      development, egress, invasion or early establishment of
                      infection in the RBC. Here, we characterise P. falciparum
                      C3H1 zinc finger 1 (PfCZIF1, $Pf3D7_1468400)$ and P.
                      falciparum C3H1 zinc finger 2 (PfCZIF2, $Pf3D7_0818100)$
                      which we identified as the only C3H1-type zinc finger
                      proteins with peak expression at schizogony. Previous
                      studies reported that antibodies against PfCZIF1 inhibit
                      merozoite invasion, suggesting this protein may have a
                      potential role during RBC invasion. We show using C-terminal
                      truncations and gene knockouts of each of Pfczif1 and
                      Pfczif2 that neither are essential for blood stage growth.
                      However, they could not both be knocked out simultaneously,
                      suggesting that at least one is needed for parasite growth
                      in vitro. Immunofluorescence localisation of PfCZIF1 and
                      PfCZIF2 indicated that both proteins occur in discrete foci
                      on the periphery of the parasite’s cytosol and biochemical
                      assays suggest they are peripherally associated to a
                      membrane. Transcriptomic analyses for the C-terminal
                      truncation mutants reveal no significant expression
                      perturbations with PfCZIF1 truncation. However, modification
                      of PfCZIF2 appears to modify the expression for some
                      exported proteins including PfKAHRP. This study does not
                      support a role for PfCZIF1 or PfCZIF2 in merozoite invasion
                      of the RBC and suggests that these proteins may help
                      regulate the expression of proteins exported into the RBC
                      cytosol after merozoite invasion.},
      cin          = {CSSB-BNITM-TG},
      ddc          = {610},
      cid          = {I:(DE-H253)CSSB-BNITM-TG-20210520},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36400305},
      UT           = {WOS:001012767000001},
      doi          = {10.1016/j.ijpara.2022.09.008},
      url          = {https://bib-pubdb1.desy.de/record/601563},
}