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@ARTICLE{WichersMisterek:601561,
      author       = {Wichers-Misterek, Jan Stephan and Binder, Annika M. and
                      Mesen-Ramirez, Paolo and Dorner, Lilian Patrick and Safavi,
                      Soraya and Fuchs, Gwendolin and Lenz, Tobias L. and
                      Bachmann, Anna and Wilson, Danny and Frischknecht, Friedrich
                      and Gilberger, Tim},
      title        = {{A} {M}icrotubule-{A}ssociated {P}rotein {I}s {E}ssential
                      for {M}alaria {P}arasite {T}ransmission},
      journal      = {mBio},
      volume       = {14},
      number       = {1},
      issn         = {2161-2129},
      address      = {Washington, DC},
      publisher    = {American Society for Microbiology},
      reportid     = {PUBDB-2024-00270},
      pages        = {e03318-22},
      year         = {2023},
      note         = {L.P.D., and F.F. were supported by DFG (SPP 2332, Physics
                      of Parasitism; FR 2140 13-1), SFB 1129, and FR 2140/10-1.
                      T.L.L. was supported by the DFG, 437857095. This work was
                      partially supported by the German Research Foundation
                      (SPP2225) and the HamburgX project grant. D.W. is supported
                      by the Humboldt Foundation.},
      abstract     = {Mature gametocytes of Plasmodium falciparum display a
                      banana (falciform) shape conferred by a complex array of
                      subpellicular microtubules (SPMT) associated with the inner
                      membrane complex (IMC). Microtubule-associated proteins
                      (MAPs) define MT populations and modulate interaction with
                      pellicular components. Several MAPs have been identified in
                      Toxoplasma gondii, and homologues can be found in the
                      genomes of Plasmodium species, but the function of these
                      proteins for asexual and sexual development of malaria
                      parasites is still unknown. Here, we identified a novel
                      subpellicular MAP, termed SPM3, that is conserved within the
                      genus Plasmodium, especially within the subgenus Laverania,
                      but absent in other Apicomplexa. Conditional knockdown and
                      targeted gene disruption of Pfspm3 in Plasmodium falciparum
                      cause severe morphological defects during gametocytogenesis,
                      leading to round, nonfalciform gametocytes with an aberrant
                      SPMT pattern. In contrast, Pbspm3 knockout in Plasmodium
                      berghei, a species with round gametocytes, caused no defect
                      in gametocytogenesis, but sporozoites displayed an aberrant
                      motility and a dramatic defect in invasion of salivary
                      glands, leading to a decreased efficiency in transmission.
                      Electron microscopy revealed a dissociation of the SPMT from
                      the IMC in Pbspm3 knockout parasites, suggesting a function
                      of SPM3 in anchoring MTs to the IMC. Overall, our results
                      highlight SPM3 as a pellicular component with essential
                      functions for malaria parasite transmission.},
      cin          = {CSSB-BNITM-TG},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-BNITM-TG-20210520},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project
                      G:(GEPRIS)437857095 - Evolutionäre Genetik und Dynamiken in
                      der Antigenpräsentation und -erkennung des adaptiven
                      Immunsystems (437857095) / DFG project G:(GEPRIS)441960173 -
                      SPP 2332: Physik des Parasitismus (441960173) / DFG project
                      G:(GEPRIS)531703706 - Entschlüsselung der molekularen
                      Mechanismen des Austritts von Orientia tsutsugamushi aus der
                      Wirtszelle (531703706)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)437857095 /
                      G:(GEPRIS)441960173 / G:(GEPRIS)531703706},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36625655},
      UT           = {WOS:000912135300001},
      doi          = {10.1128/mbio.03318-22},
      url          = {https://bib-pubdb1.desy.de/record/601561},
}