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@ARTICLE{Soh:600224,
      author       = {Soh, Timothy K. and Pfefferle, Susanne and Wurr, Stephanie
                      and von Possel, Ronald and Oestereich, Lisa and Rieger, Toni
                      and Uetrecht, Charlotte and Rosenthal, Maria and Bosse, Jens
                      Bernhard},
      title        = {{A} validated protocol to {UV}-inactivate {SARS}-{C}o{V}-2
                      and herpesvirus-infected cells},
      journal      = {PLOS ONE},
      volume       = {18},
      number       = {5},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {PUBDB-2023-07798},
      pages        = {e0274065 -},
      year         = {2023},
      abstract     = {Downstream analysis of virus-infected cell samples, such as
                      reverse transcription polymerase chain reaction (RT PCR) or
                      mass spectrometry, often needs to be performed at lower
                      biosafety levels than their actual cultivation, and thus the
                      samples require inactivation before they can be transferred.
                      Common inactivation methods involve chemical crosslinking
                      with formaldehyde or denaturing samples with strong
                      detergents, such as sodium dodecyl sulfate. However, these
                      protocols destroy the protein quaternary structure and
                      prevent the analysis of protein complexes, albeit through
                      different chemical mechanisms. This often leads to studies
                      being performed in over-expression or surrogate model
                      systems. To address this problem, we generated a protocol
                      that achieves the inactivation of infected cells through
                      ultraviolet (UV) irradiation. UV irradiation damages viral
                      genomes and crosslinks nucleic acids to proteins but leaves
                      the overall structure of protein complexes mostly intact.
                      Protein analysis can then be performed from intact cells
                      without biosafety containment. While UV treatment protocols
                      have been established to inactivate viral solutions, a
                      protocol was missing to inactivate crude infected cell
                      lysates, which heavily absorb light. In this work, we
                      develop and validate a UV inactivation protocol for
                      SARS-CoV-2, HSV-1, and HCMV-infected cells. A fluence of
                      10,000 mJ/cm2 with intermittent mixing was sufficient to
                      completely inactivate infected cells, as demonstrated by the
                      absence of viral replication even after three sequential
                      passages of cells inoculated with the treated material. The
                      herein described protocol should serve as a reference for
                      inactivating cells infected with these or similar viruses
                      and allow for the analysis of protein quaternary structure
                      from bona fide infected cells.},
      cin          = {CSSB-MHH-JB / CSSB-LIV/DESY-CU / FS-CS},
      ddc          = {610},
      cid          = {I:(DE-H253)CSSB-MHH-JB-20210520 /
                      $I:(DE-H253)CSSB-LIV_DESY-CU-20220525$ /
                      I:(DE-H253)FS-CS-20210408},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37163509},
      UT           = {WOS:001024735700001},
      doi          = {10.1371/journal.pone.0274065},
      url          = {https://bib-pubdb1.desy.de/record/600224},
}