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000599330 1001_ $$0P:(DE-H253)PIP1080980$$aMinniberger, Sonja$$b0
000599330 245__ $$aAsymmetry and Ion Selectivity Properties of Bacterial Channel NaK Mutants Derived from Ionotropic Glutamate Receptors
000599330 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2023
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000599330 520__ $$aIonotropic glutamate receptors are ligand-gated cation channels that play essential roles in the excitatory synaptic transmission throughout the central nervous system. A number of open-pore structures of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic-acid (AMPA)-type glutamate receptors became available recently by cryo-electron microscopy (cryo-EM). These structures provide valuable insights into the conformation of the selectivity filter (SF), the part of the ion channel that determines the ion selectivity. Nonetheless, due to the moderate resolution of the cryo-EM structures, detailed information such as ion occupancy of monovalent and divalent cations as well as positioning of the side-chains in the SF is still missing. Here, in an attempt to obtain high-resolution information about glutamate receptor SFs, we incorporated partial SF sequences of the AMPA and kainate receptors into the bacterial tetrameric cation channel NaK, which served as a structural scaffold. We determined a series of X-ray structures of NaK-CDI, NaK-SDI and NaK-SELM mutants at 1.42–2.10 Å resolution, showing distinct ion occupation of different monovalent cations. Molecular dynamics (MD) simulations of NaK-CDI indicated the channel to be conductive to monovalent cations, which agrees well with our electrophysiology recordings. Moreover, previously unobserved structural asymmetry of the SF was revealed by the X-ray structures and MD simulations, implying its importance in ion non-selectivity of tetrameric cation channels.
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000599330 7001_ $$aAbdolvand, Saeid$$b1
000599330 7001_ $$aBraunbeck, Sebastian$$b2
000599330 7001_ $$0P:(DE-HGF)0$$aSun, Han$$b3$$eCorresponding author
000599330 7001_ $$0P:(DE-HGF)0$$aPlested, Andrew J. R.$$b4$$eCorresponding author
000599330 773__ $$0PERI:(DE-600)1355192-9$$a10.1016/j.jmb.2023.167970$$gVol. 435, no. 6, p. 167970 -$$n6$$p167970$$tJournal of molecular biology$$v435$$x0022-2836$$y2023
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