TY  - JOUR
AU  - Wichers, Jan Stephan
AU  - Wunderlich, Juliane
AU  - Heincke, Dorothee
AU  - Pazicky, Samuel
AU  - Strauss, Jan
AU  - Schmitt, Marius
AU  - Kimmel, Jessica
AU  - Wilcke, Louisa
AU  - Scharf, Sarah
AU  - Thien, Heidrun
AU  - Burda, Paul-Christian
AU  - Spielmann, Tobias
AU  - Loew, Christian
AU  - Filarsky, Michael
AU  - Bachmann, Anna
AU  - Gilberger, Tim W.
TI  - Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum
JO  - Cellular microbiology
VL  - 23
IS  - 9
SN  - 1462-5814
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PUBDB-2023-05635
SP  - e13341
PY  - 2021
N1  - Deutsche Forschungsgemeinschaft, Grant/Award Number: BA 5213/3-1; Jürgen Manchot Stiftung; Landesforschungsförderung Hamburg, Grant/Award Number: LFF FV-69;Partnership of Universität Hamburg and DESY (PIER), Grant/Award Number: PIF-2018-87
AB  - The inner membrane complex (IMC) is a defining feature of apicomplexan parasites, which confers stability and shape to the cell, functions as a scaffolding compartment during the formation of daughter cells and plays an important role in motility and invasion during different life cycle stages of these single-celled organisms. To explore the IMC proteome of the malaria parasite Plasmodium falciparum we applied a proximity-dependent biotin identification (BioID)-based proteomics approach, using the established IMC marker protein Photosensitized INA-Labelled protein 1 (PhIL1) as bait in asexual blood-stage parasites. Subsequent mass spectrometry-based peptide identification revealed enrichment of 12 known IMC proteins and several uncharacterized candidate proteins. We validated nine of these previously uncharacterized proteins by endogenous GFP-tagging. Six of these represent new IMC proteins, while three proteins have a distinct apical localization that most likely represents structures described as apical annuli in Toxoplasma gondii. Additionally, various Kelch13 interacting candidates were identified, suggesting an association of the Kelch13 compartment and the IMC in schizont and merozoite stages. This work extends the number of validated IMC proteins in the malaria parasite and reveals for the first time the existence of apical annuli proteins in P. falciparum. Additionally, it provides evidence for a spatial association between the Kelch13 compartment and the IMC in late blood-stage parasites.
LB  - PUB:(DE-HGF)16
C6  - pmid:33830607
UR  - <Go to ISI:>//WOS:000651009000001
DO  - DOI:10.1111/cmi.13341
UR  - https://bib-pubdb1.desy.de/record/594081
ER  -