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@ARTICLE{Mehrabi:582469,
      author       = {Mehrabi, Pedram and Sung, Sihyun and Stetten, David von and
                      Prester, Andreas and Hatton, Caitlin E. and Kleine-Döpke,
                      Stephan and Berkes, Alexander and Gore, Gargi and Leimkohl,
                      Jan-Philipp and Schikora, Hendrik and Kollewe, Martin and
                      Rohde, Holger and Wilmanns, Matthias and Tellkamp, Friedjof
                      and Schulz, Eike},
      title        = {{M}illisecond cryo-trapping by the spitrobot crystal
                      plunger simplifies time-resolved crystallography},
      journal      = {Nature Communications},
      volume       = {14},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {PUBDB-2023-02014},
      pages        = {2365},
      year         = {2023},
      abstract     = {We introduce the spitrobot, a protein crystal plunger,
                      enabling reaction quenching via cryo-trapping with a
                      time-resolution in the millisecond range. Protein crystals
                      are mounted on canonical micromeshes on an electropneumatic
                      piston, where the crystals are kept in a humidity and
                      temperature-controlled environment, then reactions are
                      initiated via the liquid application method (LAMA) and
                      plunging into liquid nitrogen is initiated after an
                      electronically set delay time to cryo-trap intermediate
                      states. High-magnification images are automatically recorded
                      before and after droplet deposition, prior to plunging. The
                      SPINE-standard sample holder is directly plunged into a
                      storage puck, enabling compatibility with high-throughput
                      infrastructure. Here we demonstrate binding of glucose and
                      2,3-butanediol in microcrystals of xylose isomerase, and of
                      avibactam and ampicillin in microcrystals of the extended
                      spectrum beta-lactamase CTX-M-14. We also trap reaction
                      intermediates and conformational changes in macroscopic
                      crystals of tryptophan synthase to demonstrate that the
                      spitrobot enables insight into catalytic events.},
      cin          = {MPSD / CFEL-SSUMP / EMBL},
      ddc          = {500},
      cid          = {I:(DE-H253)MPSD-20120731 / I:(DE-H253)CFEL-SSUMP-20160915 /
                      I:(DE-H253)EMBL-20120731},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)P-P13-20150101 / EXP:(DE-H253)P-P14-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37185266},
      UT           = {WOS:001003181400017},
      doi          = {10.1038/s41467-023-37834-w},
      url          = {https://bib-pubdb1.desy.de/record/582469},
}