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@ARTICLE{Shen:582122,
      author       = {Shen, Chen and Woelk, Christian and Kikhney, Alexey G. and
                      Torres, Jaume and Surya, Wahyu and Harvey, Richard and
                      Bello, Gianluca},
      title        = {{A}bsolute scattering length density profile of liposome
                      bilayers obtained by {SAXS} combined with {GIXOS} - a tool
                      to determine model biomembrane structure},
      reportid     = {PUBDB-2023-01812},
      year         = {2022},
      abstract     = {Lipid membranes play an essential role in biology, acting
                      as host matrices for biomolecules like proteins and
                      facilitating their functions. Their structures, and
                      structural responses to physiologically relevant
                      interactions, i.e. with membrane proteins, provide key
                      information for understanding biophysical mechanisms. Hence,
                      there is a crucial need of methods to understand the effects
                      of membrane host molecules on the lipid bilayer structure.
                      Here, we present a purely experimental method for obtaining
                      the absolute scattering length density (SLD) profile and the
                      area per lipid of liposomal bilayers, by aiding the analysis
                      of small angle X-ray scattering (SAXS) data with the volume
                      of bare headgroups obtained from fast (20-120s) grazing
                      incidence off-specular scattering (GIXOS) data from
                      monolayers of the same model membrane lipid composition. The
                      GIXOS data experimentally demonstrate that the variation of
                      the bare headgroup volume upon lipid packing density change
                      is small enough to allow its usage as a reference value
                      without knowing the lipid packing stage in a bilayer. This
                      approach also bares the advantage that the reference volume
                      is obtained at the same aqueous environment as used for the
                      model membrane bilayers. We demonstrate the validity of this
                      method using several typical membrane compositions, as well
                      as one example of a phospholipid membrane with an
                      incorporated transmembrane peptide. This methodology allows
                      to obtain absolute scale values rather than relative scale
                      by using solely X-ray-based instrumentation, retaining a
                      similar resolution of SAXS experiments. The presented method
                      has high potential to understand structural effects of
                      membrane proteins on the biomembrane structure.},
      cin          = {DOOR ; HAS-User / FS-PET-D},
      cid          = {I:(DE-H253)HAS-User-20120731 /
                      I:(DE-H253)FS-PET-D-20190712},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)P-P08-20150101},
      typ          = {PUB:(DE-HGF)25},
      doi          = {10.1101/2022.12.13.520277},
      url          = {https://bib-pubdb1.desy.de/record/582122},
}