000582122 001__ 582122
000582122 005__ 20250724152239.0
000582122 0247_ $$2doi$$a10.1101/2022.12.13.520277
000582122 0247_ $$2datacite_doi$$a10.3204/PUBDB-2023-01812
000582122 0247_ $$2altmetric$$aaltmetric:140175437
000582122 0247_ $$2openalex$$aopenalex:W4311468498
000582122 037__ $$aPUBDB-2023-01812
000582122 041__ $$aEnglish
000582122 1001_ $$0P:(DE-H253)PIP1014115$$aShen, Chen$$b0$$udesy
000582122 245__ $$aAbsolute scattering length density profile of liposome bilayers obtained by SAXS combined with GIXOS - a tool to determine model biomembrane structure
000582122 260__ $$c2022
000582122 3367_ $$0PUB:(DE-HGF)25$$2PUB:(DE-HGF)$$aPreprint$$bpreprint$$mpreprint$$s1682682105_2847755
000582122 3367_ $$2ORCID$$aWORKING_PAPER
000582122 3367_ $$028$$2EndNote$$aElectronic Article
000582122 3367_ $$2DRIVER$$apreprint
000582122 3367_ $$2BibTeX$$aARTICLE
000582122 3367_ $$2DataCite$$aOutput Types/Working Paper
000582122 520__ $$aLipid membranes play an essential role in biology, acting as host matrices for biomolecules like proteins and facilitating their functions. Their structures, and structural responses to physiologically relevant interactions, i.e. with membrane proteins, provide key information for understanding biophysical mechanisms. Hence, there is a crucial need of methods to understand the effects of membrane host molecules on the lipid bilayer structure. Here, we present a purely experimental method for obtaining the absolute scattering length density (SLD) profile and the area per lipid of liposomal bilayers, by aiding the analysis of small angle X-ray scattering (SAXS) data with the volume of bare headgroups obtained from fast (20-120s) grazing incidence off-specular scattering (GIXOS) data from monolayers of the same model membrane lipid composition. The GIXOS data experimentally demonstrate that the variation of the bare headgroup volume upon lipid packing density change is small enough to allow its usage as a reference value without knowing the lipid packing stage in a bilayer. This approach also bares the advantage that the reference volume is obtained at the same aqueous environment as used for the model membrane bilayers. We demonstrate the validity of this method using several typical membrane compositions, as well as one example of a phospholipid membrane with an incorporated transmembrane peptide. This methodology allows to obtain absolute scale values rather than relative scale by using solely X-ray-based instrumentation, retaining a similar resolution of SAXS experiments. The presented method has high potential to understand structural effects of membrane proteins on the biomembrane structure.
000582122 536__ $$0G:(DE-HGF)POF4-633$$a633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633)$$cPOF4-633$$fPOF IV$$x0
000582122 536__ $$0G:(DE-HGF)POF4-6G3$$a6G3 - PETRA III (DESY) (POF4-6G3)$$cPOF4-6G3$$fPOF IV$$x1
000582122 588__ $$aDataset connected to CrossRef
000582122 693__ $$0EXP:(DE-H253)P-P08-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P08-20150101$$aPETRA III$$fPETRA Beamline P08$$x0
000582122 7001_ $$0P:(DE-H253)PIP1020591$$aWoelk, Christian$$b1
000582122 7001_ $$aKikhney, Alexey G.$$b2
000582122 7001_ $$00000-0002-1282-9727$$aTorres, Jaume$$b3
000582122 7001_ $$00000-0001-9240-371X$$aSurya, Wahyu$$b4
000582122 7001_ $$0P:(DE-H253)PIP1081540$$aHarvey, Richard$$b5$$eCorresponding author
000582122 7001_ $$0P:(DE-H253)PIP1093761$$aBello, Gianluca$$b6$$eCorresponding author
000582122 773__ $$a10.1101/2022.12.13.520277
000582122 8564_ $$uhttps://bib-pubdb1.desy.de/record/582122/files/2022.12.13.520277v1.full.pdf$$yOpenAccess
000582122 8564_ $$uhttps://bib-pubdb1.desy.de/record/582122/files/2022.12.13.520277v1.full.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000582122 909CO $$ooai:bib-pubdb1.desy.de:582122$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000582122 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1014115$$aDeutsches Elektronen-Synchrotron$$b0$$kDESY
000582122 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1014115$$aExternal Institute$$b0$$kExtern
000582122 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1020591$$aExternal Institute$$b1$$kExtern
000582122 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1081540$$aExternal Institute$$b5$$kExtern
000582122 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1093761$$aEuropean Molecular Biology Laboratory$$b6$$kEMBL
000582122 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1093761$$aExternal Institute$$b6$$kExtern
000582122 9131_ $$0G:(DE-HGF)POF4-633$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vLife Sciences – Building Blocks of Life: Structure and Function$$x0
000582122 9131_ $$0G:(DE-HGF)POF4-6G3$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vPETRA III (DESY)$$x1
000582122 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000582122 915__ $$0LIC:(DE-HGF)CCBYNCND4$$2HGFVOC$$aCreative Commons Attribution-NonCommercial-NoDerivs CC BY-NC-ND 4.0
000582122 9201_ $$0I:(DE-H253)HAS-User-20120731$$kDOOR ; HAS-User$$lDOOR-User$$x0
000582122 9201_ $$0I:(DE-H253)FS-PET-D-20190712$$kFS-PET-D$$lExperimentebetreuung PETRA III$$x1
000582122 980__ $$apreprint
000582122 980__ $$aVDB
000582122 980__ $$aUNRESTRICTED
000582122 980__ $$aI:(DE-H253)HAS-User-20120731
000582122 980__ $$aI:(DE-H253)FS-PET-D-20190712
000582122 9801_ $$aFullTexts