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@INPROCEEDINGS{Engelbert:580542,
      author       = {Engelbert, Karin and Kheirkhah, Tolue and Mueller, Henri
                      and Deffur, Charlotte and Junne, Stefan and Hammel, Joerg
                      and Briesen, Heiko and Neubauer, Peter and Meyer, Vera},
      title        = {{C}ontrolling macromorphologies of {A}spergillus niger
                      during high and low shear stress bioreactor cultivation},
      reportid     = {PUBDB-2023-01302},
      year         = {2023},
      abstract     = {Submersed cultivation of filamentous fungi is widely used
                      in fungal biotechnology. The formation of different
                      macromorphologies, however, range from dispersed mycelia
                      over loose clumps to dense pellets, and thus limits
                      productivity with shear stress as one of the main
                      influencing parameters. In this study, seed cultures with
                      defined macromorphologies of the cell factory Aspergillus
                      niger were exposed to high shear stress in stirred-tank
                      (STR) and low shear stress in rocking-motion bioreactors
                      (RMB). Talcum microparticles at 1 and 10 g L-1 were added to
                      the seed cultures to achieve pellet populations with
                      controlled diameter sizes. Physiological and morphological
                      data were comprehensively investigated with high-throughput
                      2D image analysis and 3D synchrotron radiation based
                      micro-computed tomography. This approach allowed us to
                      determine the distribution of spore agglomerates, pellets
                      and dispersed mycelia as well as hyphal densities and total
                      hyphal lengths. Our data show that high shear stress in STR
                      leads to breakage of pellets right after the stirrer was
                      switched on. The mechanical stress from stirring also
                      hindered pellets from surpassing a certain diameter during
                      cultivation. In contrast, pellet size increased constantly
                      until glucose was limited during RMB cultivations with
                      largest macromorphological changes during the exponential
                      growth phase. This work will allow us to estimate hyphal
                      growth rates and pellet breakage as a function of shear
                      stress for the first time and will furthermore pave the way
                      for better understanding of cell-bioreactor interactions,
                      and thus morphology-optimised cultivation processes.},
      month         = {Mar},
      date          = {2023-03-05},
      organization  = {16th European Conference on Fungal
                       Genetics, Innsbruck (Germany), 5 Mar
                       2023 - 8 Mar 2023},
      cin          = {DOOR ; HAS-User / Hereon / TUM},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)Hereon-20210428 /
                      I:(DE-588b)36241-4},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20211150
                      (I-20211150)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20211150},
      experiment   = {EXP:(DE-H253)P-P05-20150101},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://bib-pubdb1.desy.de/record/580542},
}