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000580542 037__ $$aPUBDB-2023-01302
000580542 041__ $$aEnglish
000580542 1001_ $$0P:(DE-H253)PIP1100833$$aEngelbert, Karin$$b0
000580542 1112_ $$a16th European Conference on Fungal Genetics$$cInnsbruck$$d2023-03-05 - 2023-03-08$$gECFG16$$wGermany
000580542 245__ $$aControlling macromorphologies of Aspergillus niger during high and low shear stress bioreactor cultivation
000580542 260__ $$c2023
000580542 3367_ $$033$$2EndNote$$aConference Paper
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000580542 520__ $$aSubmersed cultivation of filamentous fungi is widely used in fungal biotechnology. The formation of different macromorphologies, however, range from dispersed mycelia over loose clumps to dense pellets, and thus limits productivity with shear stress as one of the main influencing parameters. In this study, seed cultures with defined macromorphologies of the cell factory Aspergillus niger were exposed to high shear stress in stirred-tank (STR) and low shear stress in rocking-motion bioreactors (RMB). Talcum microparticles at 1 and 10 g L-1 were added to the seed cultures to achieve pellet populations with controlled diameter sizes. Physiological and morphological data were comprehensively investigated with high-throughput 2D image analysis and 3D synchrotron radiation based micro-computed tomography. This approach allowed us to determine the distribution of spore agglomerates, pellets and dispersed mycelia as well as hyphal densities and total hyphal lengths. Our data show that high shear stress in STR leads to breakage of pellets right after the stirrer was switched on. The mechanical stress from stirring also hindered pellets from surpassing a certain diameter during cultivation. In contrast, pellet size increased constantly until glucose was limited during RMB cultivations with largest macromorphological changes during the exponential growth phase. This work will allow us to estimate hyphal growth rates and pellet breakage as a function of shear stress for the first time and will furthermore pave the way for better understanding of cell-bioreactor interactions, and thus morphology-optimised cultivation processes.
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000580542 7001_ $$0P:(DE-HGF)0$$aKheirkhah, Tolue$$b1
000580542 7001_ $$0P:(DE-H253)PIP1092635$$aMueller, Henri$$b2
000580542 7001_ $$0P:(DE-H253)PIP1100576$$aDeffur, Charlotte$$b3
000580542 7001_ $$aJunne, Stefan$$b4
000580542 7001_ $$0P:(DE-H253)PIP1008646$$aHammel, Joerg$$b5
000580542 7001_ $$0P:(DE-H253)PIP1092638$$aBriesen, Heiko$$b6$$eCorresponding author
000580542 7001_ $$0P:(DE-HGF)0$$aNeubauer, Peter$$b7
000580542 7001_ $$0P:(DE-HGF)0$$aMeyer, Vera$$b8$$eCorresponding author
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000580542 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1100833$$a Technische Universität Berlin$$b0
000580542 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Technische Universität Berlin$$b1
000580542 9101_ $$0I:(DE-588b)36241-4$$6P:(DE-H253)PIP1092635$$aTechnische Universität München$$b2$$kTUM
000580542 9101_ $$0I:(DE-588b)36241-4$$6P:(DE-H253)PIP1100576$$aTechnische Universität München$$b3$$kTUM
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000580542 9101_ $$0I:(DE-588b)36241-4$$6P:(DE-H253)PIP1092638$$aTechnische Universität München$$b6$$kTUM
000580542 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Technische Universität Berlin$$b7
000580542 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Technische Universität Berlin$$b8
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000580542 9141_ $$y2023
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